Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

View all BCL11B Antibodies »

Product Specifications

Immunogen

A synthetic peptide which maps to a region between residues 500 and 550 of human B-cell CLL/lymphoma 11B using the numbering given in Swiss-Prot entry Q9C0K0 (GeneID 64919).

Specificity

NB600-263 is specific for human Bcl-11b protein.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for BCL11B Antibody - BSA Free

Western Blot: BCL11B Antibody [NB600-263]

Western Blot: BCL11B Antibody [NB600-263]

Western Blot: BCL11B Antibody [NB600-263] - Detection of Human Bcl11b by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Bcl11b antibody NB600-263 used for WB at 0.066 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunohistochemistry-Paraffin: BCL11B Antibody [NB600-263]

Immunohistochemistry-Paraffin: BCL11B Antibody [NB600-263]

Immunohistochemistry-Paraffin: BCL11B Antibody [NB600-263] - Section of human stomach carcinoma. Antibody: Affinity purified rabbit anti-Bcl11b used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Immunoprecipitation: BCL11B Antibody [NB600-263]

Immunoprecipitation: BCL11B Antibody [NB600-263]

Immunoprecipitation: BCL11B Antibody [NB600-263] - Detection of Human Bcl11b on HeLa whole cell lysate using NB600-263. Bcl11b was also immunoprecipitated using affinity purified rabbit anti-Bcl11b antibodies NB600-262 and NB600-264.

Applications for BCL11B Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:500 - 1:2000

Immunohistochemistry-Paraffin

1:500 - 1:2000

Immunoprecipitation

1-5 ug/mg lysate

Western Blot

1:5000-1:15000
Application Notes
Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: BCL11B

This gene encodes a C2H2-type zinc finger protein and is closely related to BCL11A, a gene whose translocation may be associated with B-cell malignancies. The specific function of this gene has not yet been determined. Two alternatively spliced transcript variants, which encode distinct isoforms, have been reported.

Alternate Names

B-cell CLL/lymphoma 11B, B-cell CLL/lymphoma 11B (zinc finger protein), B-cell CLL/lymphoma 11B/T-cell receptor delta constant region fusion protein, B-cell lymphoma/leukaemia 11B, B-cell lymphoma/leukemia 11B, BCL-11B, BCL11B/TRDC fusion, COUP-TF-interacting protein 2, CTIP-2, CTIP2ZNF856B, hRit1, hRIT1-alpha, Radiation-induced tumor suppressor gene 1 protein, RIT1, zinc finger protein hRit1 alpha

Entrez Gene IDs

64919 (Human)

Gene Symbol

BCL11B

UniProt

Q9C0K0 (Human)

Additional BCL11B Products

Product Documents for BCL11B Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for BCL11B Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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