Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all BCL11B Antibodies »

Product Specifications

Immunogen

A synthetic peptide which maps to a region between residues 500 and 550 of human B-cell CLL/lymphoma 11B using the numbering given in Swiss-Prot entry Q9C0K0 (GeneID 64919).

Specificity

NB600-263 is specific for human Bcl-11b protein.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for BCL11B Antibody

Western Blot: BCL11B Antibody [NB600-263]

Western Blot: BCL11B Antibody [NB600-263]

Western Blot: BCL11B Antibody [NB600-263] - Detection of Human Bcl11b by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Bcl11b antibody NB600-263 used for WB at 0.066 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunohistochemistry-Paraffin: BCL11B Antibody [NB600-263]

Immunohistochemistry-Paraffin: BCL11B Antibody [NB600-263]

Immunohistochemistry-Paraffin: BCL11B Antibody [NB600-263] - Section of human stomach carcinoma. Antibody: Affinity purified rabbit anti-Bcl11b used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Immunoprecipitation: BCL11B Antibody [NB600-263]

Immunoprecipitation: BCL11B Antibody [NB600-263]

Immunoprecipitation: BCL11B Antibody [NB600-263] - Detection of Human Bcl11b on HeLa whole cell lysate using NB600-263. Bcl11b was also immunoprecipitated using affinity purified rabbit anti-Bcl11b antibodies NB600-262 and NB600-264.

Applications for BCL11B Antibody

Application
Recommended Usage

Immunohistochemistry

1:500 - 1:2000

Immunohistochemistry-Paraffin

1:500 - 1:2000

Immunoprecipitation

1-5 ug/mg lysate

Western Blot

1:5000-1:15000
Application Notes
Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: BCL11B

This gene encodes a C2H2-type zinc finger protein and is closely related to BCL11A, a gene whose translocation may be associated with B-cell malignancies. The specific function of this gene has not yet been determined. Two alternatively spliced transcript variants, which encode distinct isoforms, have been reported.

Alternate Names

B-cell CLL/lymphoma 11B, B-cell CLL/lymphoma 11B (zinc finger protein), B-cell CLL/lymphoma 11B/T-cell receptor delta constant region fusion protein, B-cell lymphoma/leukaemia 11B, B-cell lymphoma/leukemia 11B, BCL-11B, BCL11B/TRDC fusion, COUP-TF-interacting protein 2, CTIP-2, CTIP2ZNF856B, hRit1, hRIT1-alpha, Radiation-induced tumor suppressor gene 1 protein, RIT1, zinc finger protein hRit1 alpha

Entrez Gene IDs

64919 (Human)

Gene Symbol

BCL11B

UniProt

Q9C0K0 (Human)

Additional BCL11B Products

Product Documents for BCL11B Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for BCL11B Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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