BCR Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-19097
![Immunocytochemistry/ Immunofluorescence: BCR Antibody [NBP1-19097]](https://resources.bio-techne.com/images/products/BCR-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-19097-img0009.jpg "Immunocytochemistry/ Immunofluorescence: BCR Antibody [NBP1-19097]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot (Negative)
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residue 50 and 100 of human breakpoint cluster region using the numbering given in entry NP_004318.3 (GeneID 613).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be used as the primary antibody in a PLA assay with the following as complementing antibodies:NBP1-19098, NB200-171
Scientific Data Images for BCR Antibody - BSA Free
Immunocytochemistry/ Immunofluorescence: BCR Antibody [NBP1-19097]
Immunocytochemistry/Immunofluorescence: BCR Antibody [NBP1-19097] - Human K562 cells (contains the chromosomal translocation, t(9:22) that creates the BCR/ABL fusion gene). Antibody used at a dilution of 1:1,000 (1ug/ml). Detection: Green-fluorescent goat anti-rabbit IgG - AlexaFluor 488 used at a dilution of 1:1,000.![Flow Cytometry: BCR Antibody [NBP1-19097]](https://resources.bio-techne.com/images/products/BCR-Antibody-Flow-Cytometry-NBP1-19097-img0006.jpg "Flow Cytometry: BCR Antibody [NBP1-19097]")
Flow Cytometry: BCR Antibody [NBP1-19097]
Flow Cytometry: BCR Antibody [NBP1-19097] - Flow Cytometric Detection of BCR. 1 x 106 K562 cells were fixed, permeabilized, and stained with 0.25 mcg anti-BCR in a 150 mcl reaction. Isotype anti-KLH control (black), no antibody (red) and anti-BCR (blue).![Immunoprecipitation: BCR Antibody [NBP1-19097]](https://resources.bio-techne.com/images/products/BCR-Antibody-Immunoprecipitation-NBP1-19097-img0010.jpg "Immunoprecipitation: BCR Antibody [NBP1-19097]")
Immunoprecipitation: BCR Antibody [NBP1-19097]
Immunoprecipitation: BCR Antibody [NBP1-19097] - Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Affinity purified rabbit anti-BCR antibody used for IP at 10 mcg/mg lysate. BCR was also immunoprecipitated by rabbit anti-BCR antibody NBP1-19098, which recognizes a downstream epitopeApplications for BCR Antibody - BSA Free
Application
Recommended Usage
Flow Cytometry
0.25 mcg per 1X10e6 cells in 150 mcl volume
Immunocytochemistry/ Immunofluorescence
1:500 - 1:2000
Immunoprecipitation
10 ug/mg lysate
Application Notes
Western Blot Not recommended.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: BCR
Long Name
Breakpoint Cluster Region
Alternate Names
ALL, PHL
Entrez Gene IDs
613 (Human)
Gene Symbol
BCR
UniProt
Additional BCR Products
Product Documents for BCR Antibody - BSA Free
Product Specific Notices for BCR Antibody - BSA Free
This antibody can be used as the primary antibody in a PLA assay with the following as complementing antibodies:NBP1-19098, NB200-171
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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