BCR Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-19098
![Western Blot: BCR Antibody [NBP1-19098]](https://resources.bio-techne.com/images/products/BCR-Antibody-Western-Blot-NBP1-19098-img0008.jpg "Western Blot: BCR Antibody [NBP1-19098]")
Conjugate
Catalog #
Key Product Details
Validated by
Independent Antibodies, Biological Validation
Species Reactivity
Validated:
Human
Predicted:
Mouse (100%). Backed by our 100% Guarantee.
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residue 1221 and 1271 of human breakpoint cluster region using the numbering given in entry NP_004318.3 (GeneID 613).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be used as the primary antibody in a PLA assay with the following as complementing antibodies:NBP1-19097, NB100-77315
Scientific Data Images for BCR Antibody - BSA Free
![Immunocytochemistry/ Immunofluorescence: BCR Antibody [NBP1-19098]](https://resources.bio-techne.com/images/products/BCR-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-19098-img0007.jpg "Immunocytochemistry/ Immunofluorescence: BCR Antibody [NBP1-19098]")
Immunocytochemistry/ Immunofluorescence: BCR Antibody [NBP1-19098]
Immunocytochemistry/Immunofluorescence: BCR Antibody [NBP1-19098] - Human K562 cells (contains the chromosomal translocation, t(9:22) that creates the BCR/ABL fusion gene). Antibody used at a dilution of 1:1,000 (1ug/ml). Detection: Green-fluorescent goat anti-rabbit IgG - Alexa Fluor 488 used at a dilution of 1:1,000.![Flow Cytometry: BCR Antibody [NBP1-19098]](https://resources.bio-techne.com/images/products/BCR-Antibody-Flow-Cytometry-NBP1-19098-img0006.jpg "Flow Cytometry: BCR Antibody [NBP1-19098]")
Flow Cytometry: BCR Antibody [NBP1-19098]
Flow Cytometry: BCR Antibody [NBP1-19098] - Flow Cytometric Detection of BCR. 1 x 106 K562 cells were fixed, permeabilized, and stained with 0.25 mcg anti-BCR in a 150 mcl reaction. Isotype anti-KLH control (red), no antibody (blue) and anti-BCR (black).Applications for BCR Antibody - BSA Free
Application
Recommended Usage
Flow Cytometry
0.5 ug/10^6 cells in 150 ul
Immunocytochemistry/ Immunofluorescence
1:500-1:2000
Immunoprecipitation
10 ug/mg lysate
Western Blot
1:2000-1:10000
Application Notes
Western blot of lysates performed using standard western blot reagents and 4-8% SDS-PAGE.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: BCR
Long Name
Breakpoint Cluster Region
Alternate Names
ALL, PHL
Entrez Gene IDs
613 (Human)
Gene Symbol
BCR
UniProt
Additional BCR Products
Product Documents for BCR Antibody - BSA Free
Product Specific Notices for BCR Antibody - BSA Free
This antibody can be used as the primary antibody in a PLA assay with the following as complementing antibodies:NBP1-19097, NB100-77315
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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