BCR Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-19098

Novus Biologicals, part of Bio-Techne

Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%). Backed by our 100% Guarantee.

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

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Product Summary for BCR Antibody

Immunogen

The immunogen recognized by this antibody maps to a region between residue 1221 and 1271 of human breakpoint cluster region using the numbering given in entry NP_004318.3 (GeneID 613).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be used as the primary antibody in a PLA assay with the following as complementing antibodies:NBP1-19097, NB100-77315

Scientific Data Images for BCR Antibody

Western Blot: BCR Antibody [NBP1-19098]

Western Blot: BCR Antibody [NBP1-19098]

Western Blot: BCR Antibody [NBP1-19098] - Whole cell lysate from HeLa and 293T cells. BCR was also immunoprecipitated by rabbit anti-BCR antibody NBP1-19097.
Immunocytochemistry/ Immunofluorescence: BCR Antibody [NBP1-19098]

Immunocytochemistry/ Immunofluorescence: BCR Antibody [NBP1-19098]

Immunocytochemistry/Immunofluorescence: BCR Antibody [NBP1-19098] - Human K562 cells (contains the chromosomal translocation, t(9:22) that creates the BCR/ABL fusion gene). Antibody used at a dilution of 1:1,000 (1ug/ml). Detection: Green-fluorescent goat anti-rabbit IgG - Alexa Fluor 488 used at a dilution of 1:1,000.
Flow Cytometry: BCR Antibody [NBP1-19098]

Flow Cytometry: BCR Antibody [NBP1-19098]

Flow Cytometry: BCR Antibody [NBP1-19098] - Flow Cytometric Detection of BCR. 1 x 106 K562 cells were fixed, permeabilized, and stained with 0.25 mcg anti-BCR in a 150 mcl reaction. Isotype anti-KLH control (red), no antibody (blue) and anti-BCR (black).

Applications for BCR Antibody

Application
Recommended Usage

Flow Cytometry

0.5 ug/10^6 cells in 150 ul

Immunocytochemistry/ Immunofluorescence

1:500-1:2000

Immunoprecipitation

10 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
Western blot of lysates performed using standard western blot reagents and 4-8% SDS-PAGE.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: BCR

A reciprocal translocation between chromosomes 22 and 9 produces the Philadelphia chromosome, which is often found in patients with chronic myelogenous leukemia. The chromosome 22 breakpoint for this translocation is located within the BCR gene. The translocation produces a fusion protein which is encoded by sequence from both BCR and ABL, the gene at the chromosome 9 breakpoint. Although the BCR-ABL fusion protein has been extensively studied, the function of the normal BCR gene product is not clear. The protein has serine/threonine kinase activity and is a GTPase-activating protein for p21rac. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Long Name

Breakpoint Cluster Region

Alternate Names

ALL, PHL

Entrez Gene IDs

613 (Human)

Gene Symbol

BCR

UniProt

NP_004318.3 (Human)

Additional BCR Products

Product Documents for BCR Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for BCR Antibody

This antibody can be used as the primary antibody in a PLA assay with the following as complementing antibodies:NBP1-19097, NB100-77315

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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