Key Product Details

Species Reactivity

Validated:

Human

Cited:

Rat

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Simple Western, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 35928.11

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all BDNF Antibodies »

Product Specifications

Immunogen

Recombinant human BDNF (rhBDNF) expressed in Sf 21 insect cells.

Localization

Secreted

Specificity

Monoclonal Anti-Human BDNF will neutralize the biological activity of rhBDNF. This antibody shows < 2% cross-reactivity with rhbeta-NGF, rhNT-3 and rhNT-4 based on ELISA and immunoblotting. The antibody shows ~5% cross-reactivity with recombinant human beta-NGF and recombinant rat beta-NGF

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for BDNF Antibody (35928.11)

Immunocytochemistry/ Immunofluorescence: BDNF Antibody (35928.11) [NB120-10505]

Immunocytochemistry/ Immunofluorescence: BDNF Antibody (35928.11) [NB120-10505]

Immunocytochemistry/Immunofluorescence: BDNF Antibody (35928.11) [NB120-10505] - Human thyroid tissue was incubated with anti-BDNF antibody at a dilution of 1:100 overnight at 4C and detected with Alexa Fluor 594 Donkey anti-mouse 1:500. Nuclei stained with DAPI. ICC/IF image submitted by a verified customer review.

Applications for BDNF Antibody (35928.11)

Application
Recommended Usage

Immunohistochemistry-Paraffin

8 - 25 ug/mL

Western Blot

1 - 2 ug/mL
Application Notes
WB: This antibody detects BDNF at 300 ng/lane under non-reducing and reducing conditions. Immunohistochemistry (formalin-fixed, paraffin-embedded sections): 8-25 ug/mL using human spinal cord. This BDNF Antibody (35928.11) is validated for ICC/IF from a verified customer review.

See Simple Western Antibody Database for Simple Western validation: separated by size
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 4 using NB120-10505 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS, 5% trehalose

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: BDNF

Brain-derived neurotrophic factor (BDNF) is a member of the neurotrophin family of growth factors that includes NGF, NT-3, and NT-4. All neurotrophins have six conserved cysteine residues and share a 55% sequence identity at the amino acid level. BDNF has been shown to enhance the survival and differentiation of several classes of neurons in vitro, including neural crest and placode-derived sensory neurons, dopaminergic neurons in the substantia nigra, basal forebrain cholinergic neurons, hippocampal neurons, and retinal ganglial cells.1 BDNF is expressed within peripheral ganglia and is not restricted to neuronal target fields, raising the possibility that BDNF has paracrine or even autocrine actions on neurons as well as non-neuronal cells.2

Long Name

Brain-derived Neurotrophic Factor

Alternate Names

Abrineurin, ANON2, BULN2, Neurotrophin

Entrez Gene IDs

627 (Human)

Gene Symbol

BDNF

Additional BDNF Products

Product Documents for BDNF Antibody (35928.11)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for BDNF Antibody (35928.11)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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