Beclin 1 [p Ser234] Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-29654
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Predicted:
Bovine (100%), Canine (100%), Mouse (100%), Primate (100%), Rat (100%), Sheep (100%). Backed by our 100% Guarantee.
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser234 conjugated to KLH. Accession # Q14457. Accession # Q14457
Reactivity Notes
The antibody has been directly tested for reactivity in Western blots with recombinant HeLa and 293T cells. It is anticipated that the antibody will react with bovine, canine, mouse, non-human primate, rat, and sheep tissues based on the fact that these species have 100% homology with the amino acid sequence used as antigen.
Modification
p Ser234
Specificity
Specific for endogenous levels of the ~60 kDa beclin 1 protein phosphorylated at Ser234. Immunolabeling of the beclin 1 band is decreased in the beclin 1 alanine substitution mutant.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
60 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Beclin 1 [p Ser234] Antibody
Western Blot: Beclin 1/ATG6 [p Ser234] Antibody [NBP2-29654] - Western Blot: Beclin 1/ATG6 [p Ser234] Antibody [NBP2-29654] - Beclin 1 [p Ser234] Antibody [NBP2-29654] - flag-beclin-1 WT immunoprecipitates expressed in 293T cells showing specific immunolabeling of beclin-1 when phosphorylated at S234 (WT). This immunolabeling is decreased in the beclin-1 double alanine substitution mutant (S234A, S295A).
Applications for Beclin 1 [p Ser234] Antibody
Application
Recommended Usage
Western Blot
1:250
Application Notes
Immunolabeling of the beclin-1 band is decreased in the beclin-1 alanine substitution mutant. Additional bands may be observed in non-immunoprecipitated lysates.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Beclin 1
Long Name
Beclin1, Autophagy-related
Alternate Names
ATG6, BECN1, GT197, VPS30
Entrez Gene IDs
8678 (Human)
Gene Symbol
BECN1
UniProt
Additional Beclin 1 Products
Product Documents for Beclin 1 [p Ser234] Antibody
Product Specific Notices for Beclin 1 [p Ser234] Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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