Beclin 1 [p Ser90] Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13298
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Summary for Beclin 1 [p Ser90] Antibody
Immunogen
Carrier-protein conjugated synthetic peptide surrounding phospho Ser90 of human Beclin 1. The exact sequence is proprietary.
Modification
p Ser90
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Centrifuge briefly prior to opening.
Scientific Data Images for Beclin 1 [p Ser90] Antibody
![Western Blot: Beclin 1 [p Ser90] Antibody [NBP3-13298]](https://resources.bio-techne.com/images/products/Beclin-1-[p-Ser90]-Antibody-Western-Blot-NBP3-13298-img0001.jpg "Western Blot: Beclin 1 [p Ser90] Antibody [NBP3-13298]")
Western Blot: Beclin 1 [p Ser90] Antibody [NBP3-13298]
Western Blot: Beclin 1 [p Ser90] Antibody [NBP3-13298] - Untreated (-) and treated (+) MCF-7 whole cell extracts (60 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Beclin1 (phospho Ser90) antibody (NBP3-13298) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.Applications for Beclin 1 [p Ser90] Antibody
Application
Recommended Usage
Western Blot
1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS (pH 7), 20% Glycerol, 1% BSA
Preservative
0.025% Proclin 300
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Beclin 1
Beclin-1 encodes an evolutionarily conserved 60kDa coiled-coil protein that is expressed in human muscle, epithelial cells and neurons. In gene transfer studies, beclin 1 promotes nutrient deprivation-induced autophagy, inhibits mammary tumorigenesis, and inhibits viral replication.
Expression of the Beclin1 protein is frequently decreased in malignant breast epithelial cells. Based upon these observations, it is speculated that beclin-1 may work through induction of autophagy to negatively regulate tumorigenesis and to control viral infections. Beclin 1 may also play a role in other biological processes in which autophagy is important such as cell differentiation and nutritional stress responses.
Long Name
Beclin1, Autophagy-related
Alternate Names
ATG6, BECN1, GT197, VPS30
Gene Symbol
BECN1
Additional Beclin 1 Products
Product Documents for Beclin 1 [p Ser90] Antibody
Product Specific Notices for Beclin 1 [p Ser90] Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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