Bestrophin 1 Antibody (E6-6) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-80593

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Mouse, Porcine, Canine, Primate, Rat (Negative)

Applications

Dual RNAscope ISH-IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # E6-6

Format

Azide and BSA Free

Concentration

1 mg/ml

View all Bestrophin 1 Antibodies »

Product Specifications

Immunogen

Synthetic peptide conjugated to KLH corresponding to the C-terminus of human Bestrophin 1 (KDHMDPYWALENRDEAHS)

Reactivity Notes

Human, Primate, Porcine reactivity reported in scientific literature (PMID: 11050159).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for Bestrophin 1 Antibody (E6-6) - Azide and BSA Free

Western Blot: Bestrophin 1 Antibody (E6-6)Azide and BSA Free [NBP2-80593]

Western Blot: Bestrophin 1 Antibody (E6-6)Azide and BSA Free [NBP2-80593]

Western Blot: Bestrophin 1 Antibody (E6-6) - Azide and BSA Free [NBP2-80593] - Western blot analysis of the normal and mutant human Best1 protein in transiently transfected MDCK cells. Best1 proteins are detectable as a 68 kDa band in all transfected cells, but not in non-transfected controls (MDCK lane). Actin bands are shown to indicate equal loading of cell lysates. Image collected and cropped by CiteAb from the following publication (https://www.mdpi.com/1422-0067/14/7/15121), licensed under a CC-BY license. Image from the standard format of this antibody.
Immunocytochemistry: Bestrophin 1 Antibody (E6-6) - Azide and BSA Free [NBP2-80593]

Immunocytochemistry: Bestrophin 1 Antibody (E6-6) - Azide and BSA Free [NBP2-80593]

Immunocytochemistry: Bestrophin 1 Antibody (E6-6) - Azide and BSA Free [NBP2-80593] - Bestrophin 1 was detected in immersion fixed paraffin-embedded sections of human brain using Mouse Anti-Human Bestrophin 1 (E6-6) Monoclonal Antibody (Catalog # NB300-164) at 1:300 for 1 hour at room temperature followed by incubation with the Anti-Mouse
Immunohistochemistry: Bestrophin 1 Antibody (E6-6) - Azide and BSA Free [NBP2-80593]

Immunohistochemistry: Bestrophin 1 Antibody (E6-6) - Azide and BSA Free [NBP2-80593]

Immunohistochemistry: Bestrophin 1 Antibody (E6-6) - Azide and BSA Free [NBP2-80593] - Bestrophin 1 was detected in immersion fixed paraffin sections of human small intestine using t Mouse Anti-Human Bestrophin 1 Monoclonal Antibody (Catalog # NB300-164) at 5 ug/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse Ig
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Applications for Bestrophin 1 Antibody (E6-6) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

reported in scientific literature (PMID 30048622)

Immunohistochemistry-Paraffin

reported in scientific literature (PMID 24345323)

Western Blot

1:1000
Application Notes
In Western blot, this antibody recognizes a band at ~68 kDa representing Bestrophin. Please see protocol for treatment of cell extracts. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Bestrophin 1

Best macular dystrophy (BMD) or vitelliform macular dystrophy (VMD2), is an autosomal form of macular degeneration, characterized by a depressed light peak in the electrooculogram (EOG). It is inherited and has an early onset. Bestrophin is a 68 kDa basolateral plasma membrane protein encoded by the VMD2 gene. Bestrophin's function is still unknown, but data suggests that it is a chloride channel that plays a role in generating the altered EOG in Best disease patients. In addition, Bestrophin is a useful biochemical and histological marker of RPE (retinal pigment epithelial cells) cells.

Alternate Names

ARB, BEST, Best disease, bestrophin 1, bestrophin-1, BMD, TU15B, vitelliform macular dystrophy 2, Vitelliform macular dystrophy protein 2, VMD2RP50

Gene Symbol

BEST1

Additional Bestrophin 1 Products

Product Documents for Bestrophin 1 Antibody (E6-6) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Bestrophin 1 Antibody (E6-6) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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