beta-1,4-Galactosyltransferase 1/B4GalT1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-88654
![Immunohistochemistry-Paraffin: beta-1,4-Galactosyltransferase 1/B4GalT1 Antibody [NBP1-88654]](https://resources.bio-techne.com/images/products/beta-1-4-Galactosyltransferase-1-B4GalT1-Antibody-Immunohistochemistry-Paraffin-NBP1-88654-img0023.jpg "Immunohistochemistry-Paraffin: beta-1,4-Galactosyltransferase 1/B4GalT1 Antibody [NBP1-88654]")
Key Product Details
Validated by
Orthogonal Validation, Independent Antibodies
Species Reactivity
Human
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: LPQLVGVSTPLQGGSNSAAAIGQSSGELRTGGARPPPPLGASSQPRPGGDSSPVVDSGPGPASNLTSVPVPHTTALSLPACPEESPLLVGPMLIEFNMPVDLELVAKQNPNVKM
Reactivity Notes
Reactivity reported in scientific literature (PMID: 23511973).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for beta-1,4-Galactosyltransferase 1/B4GalT1 Antibody
![Immunohistochemistry-Paraffin: beta-1,4-Galactosyltransferase 1/B4GalT1 Antibody [NBP1-88654]](https://resources.bio-techne.com/images/products/beta-1-4-Galactosyltransferase-1-B4GalT1-Antibody-Immunohistochemistry-Paraffin-NBP1-88654-img0020.jpg "Immunohistochemistry-Paraffin: beta-1,4-Galactosyltransferase 1/B4GalT1 Antibody [NBP1-88654]")
Immunohistochemistry-Paraffin: beta-1,4-Galactosyltransferase 1/B4GalT1 Antibody [NBP1-88654]
Immunohistochemistry-Paraffin: beta-1,4-Galactosyltransferase 1/B4GalT1 Antibody [NBP1-88654] - Staining of human placenta, prostate, skeletal muscle and testis using Anti-B4GALT1 antibody NBP1-88654 (A) shows similar protein distribution across tissues to independent antibody NBP1-88655 (B).![Western Blot: beta-1,4-Galactosyltransferase 1/B4GalT1 Antibody [NBP1-88654]](https://resources.bio-techne.com/images/products/beta-1-4-Galactosyltransferase-1-B4GalT1-Antibody-Western-Blot-NBP1-88654-img0013.jpg "Western Blot: beta-1,4-Galactosyltransferase 1/B4GalT1 Antibody [NBP1-88654]")
Western Blot: beta-1,4-Galactosyltransferase 1/B4GalT1 Antibody [NBP1-88654]
Western Blot: beta-1,4-Galactosyltransferase 1/B4GalT1 Antibody [NBP1-88654] - Analysis in human cell lines HeLa and HEK293 using anti-B4GALT1 antibody. Corresponding B4GALT1 RNA-seq data are presented for the same cell lines. Loading control: anti-HSP90B1.![Immunocytochemistry/ Immunofluorescence: beta-1,4-Galactosyltransferase 1/B4GalT1 Antibody [NBP1-88654]](https://resources.bio-techne.com/images/products/beta-1-4-Galactosyltransferase-1-B4GalT1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-88654-img0007.jpg "Immunocytochemistry/ Immunofluorescence: beta-1,4-Galactosyltransferase 1/B4GalT1 Antibody [NBP1-88654]")
Immunocytochemistry/ Immunofluorescence: beta-1,4-Galactosyltransferase 1/B4GalT1 Antibody [NBP1-88654]
Immunocytochemistry/Immunofluorescence: beta-1,4-Galactosyltransferase 1/B4GalT1 Antibody [NBP1-88654] - Staining of human cell line A-431 shows localization to the Golgi apparatus. Antibody staining is shown in green.![Immunohistochemistry-Paraffin: beta-1,4-Galactosyltransferase 1/B4GalT1 Antibody [NBP1-88654]](https://resources.bio-techne.com/images/products/beta-1-4-Galactosyltransferase-1-B4GalT1-Antibody-Immunohistochemistry-Paraffin-NBP1-88654-img0019.jpg "Immunohistochemistry-Paraffin: beta-1,4-Galactosyltransferase 1/B4GalT1 Antibody [NBP1-88654]")
![Immunohistochemistry-Paraffin: beta-1,4-Galactosyltransferase 1/B4GalT1 Antibody [NBP1-88654]](https://resources.bio-techne.com/images/products/beta-1-4-Galactosyltransferase-1-B4GalT1-Antibody-Immunohistochemistry-Paraffin-NBP1-88654-img0021.jpg "Immunohistochemistry-Paraffin: beta-1,4-Galactosyltransferase 1/B4GalT1 Antibody [NBP1-88654]")
![Immunohistochemistry-Paraffin: beta-1,4-Galactosyltransferase 1/B4GalT1 Antibody [NBP1-88654]](https://resources.bio-techne.com/images/products/beta-1-4-Galactosyltransferase-1-B4GalT1-Antibody-Immunohistochemistry-Paraffin-NBP1-88654-img0022.jpg "Immunohistochemistry-Paraffin: beta-1,4-Galactosyltransferase 1/B4GalT1 Antibody [NBP1-88654]")
Applications for beta-1,4-Galactosyltransferase 1/B4GalT1 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:500 - 1:1000
Immunohistochemistry-Paraffin
1:500 - 1:1000
Western Blot
0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: beta-1,4-Galactosyltransferase 1/B4GalT1
Long Name
UDP-Gal:betaGlcNAc beta 1,4-galactosyltransferase, Polypeptide 1
Alternate Names
GGTB2
Gene Symbol
B4GALT1
Additional beta-1,4-Galactosyltransferase 1/B4GalT1 Products
Product Documents for beta-1,4-Galactosyltransferase 1/B4GalT1 Antibody
Product Specific Notices for beta-1,4-Galactosyltransferase 1/B4GalT1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...