beta 2-Microglobulin Antibody (3F9-2C2)
Novus Biologicals, part of Bio-Techne | Catalog # H00000567-M01
Key Product Details
Species Reactivity
Human
Applications
Validated:
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Proximity Ligation Assay, Western Blot
Cited:
IF/IHC
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2b Kappa Clone # 3F9-2C2
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
B2M (AAH32589, 1 a.a. ~ 119 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MSRSVALAVLALLSLSGLEAIQRTPKIQVYSRHPAENGKSNFLNCYVSGFHPSDIEVDLLKNGERIEKVEHSDLSFSKDWSFYLLYYTEFTPTEKDEYACRVNHVTLSQPKIVKWDRDM
Specificity
B2M - beta-2-microglobulin
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2b Kappa
Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Scientific Data Images for beta 2-Microglobulin Antibody (3F9-2C2)
Western Blot: beta 2-Microglobulin Antibody (3F9-2C2) [H00000567-M01]
Western Blot: beta 2-Microglobulin Antibody (3F9-2C2) [H00000567-M01] - Analysis of B2M expression in transfected 293T cell line by B2M monoclonal antibody (M01), clone 3F9-2C2.Lane 1: B2M transfected lysate(13.7 KDa).Lane 2: Non-transfected lysate.Immunocytochemistry/ Immunofluorescence: beta 2-Microglobulin Antibody (3F9-2C2) [H00000567-M01]
Immunocytochemistry/Immunofluorescence: beta 2-Microglobulin Antibody (3F9-2C2) [H00000567-M01] - Analysis of monoclonal antibody to B2M on HeLa cell. Antibody concentration 10 ug/ml.Immunohistochemistry-Paraffin: beta 2-Microglobulin Antibody (3F9-2C2) [H00000567-M01]
Immunohistochemistry-Paraffin: beta 2-Microglobulin Antibody (3F9-2C2) [H00000567-M01] - Analysis of monoclonal antibody to B2M on formalin-fixed paraffin-embedded human esophagus. Antibody concentration 3 ug/ml.Applications for beta 2-Microglobulin Antibody (3F9-2C2)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:10-1:500
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Paraffin
1:10-1:500
Western Blot
1:500
Application Notes
Antibody reactivity against recominant protein and cell lysate for WB. It has been used for IF, IHC-P, RNAi Validation and ELISA.Use in Immunohistochemistry reported in scientific literature (PMID: 24736453).
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: beta 2-Microglobulin
Alternate Names
B2M, beta 2Microglobulin
Entrez Gene IDs
567 (Human)
Gene Symbol
B2M
OMIM
109700 (Human)
UniProt
Additional beta 2-Microglobulin Products
Product Documents for beta 2-Microglobulin Antibody (3F9-2C2)
Product Specific Notices for beta 2-Microglobulin Antibody (3F9-2C2)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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