beta 2-Microglobulin Antibody (B2M/1118)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-44467
![Flow Cytometry: beta 2-Microglobulin Antibody (B2M/1118) [NBP2-44467]](https://resources.bio-techne.com/images/products/beta-2-Microglobulin-Antibody-B2M-1118-Flow-Cytometry-NBP2-44467-img0008.jpg "Flow Cytometry: beta 2-Microglobulin Antibody (B2M/1118) [NBP2-44467]")
![Western Blot: beta 2-Microglobulin Antibody (B2M/1118) [NBP2-44467]](https://resources.bio-techne.com/images/products/beta-2-Microglobulin-Antibody-B2M-1118-Western-Blot-NBP2-44467-img0009.jpg "Western Blot: beta 2-Microglobulin Antibody (B2M/1118) [NBP2-44467]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Primate
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # B2M/1118
Concentration
0.2 mg/ml
Product Summary for beta 2-Microglobulin Antibody (B2M/1118)
Immunogen
Full length recombinant human beta 2-Microglobulin protein (Uniprot: P61769)
Reactivity Notes
Non-Human primates.
Localization
Cell Surface and Extracellular (Secreted)
Marker
Renal Failure & Tumor Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Theoretical MW
12 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-47702)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for beta 2-Microglobulin Antibody (B2M/1118)
Western Blot: beta 2-Microglobulin Antibody (B2M/1118) [NBP2-44467]
Western Blot: beta 2-Microglobulin Antibody (B2M/1118) [NBP2-44467] - Western Blot Analysis of THP-1 and Raji Cell lysate using beta 2-Microglobulin Antibody (B2M/1118).![Immunocytochemistry/ Immunofluorescence: beta 2-Microglobulin Antibody (B2M/1118) [NBP2-44467]](https://resources.bio-techne.com/images/products/beta-2-Microglobulin-Antibody-B2M-1118-Immunocytochemistry-Immunofluorescence-NBP2-44467-img0007.jpg "Immunocytochemistry/ Immunofluorescence: beta 2-Microglobulin Antibody (B2M/1118) [NBP2-44467]")
Immunocytochemistry/ Immunofluorescence: beta 2-Microglobulin Antibody (B2M/1118) [NBP2-44467]
Immunocytochemistry/Immunofluorescence: beta 2-Microglobulin Antibody (B2M/1118) [NBP2-44467] - Immunofluorescent staining of HeLa cells. beta 2-Microglobulin Mouse Monoclonal Antibody (B2M/1118) followed by goat anti-Mouse IgG-CF488 (Green).![Immunohistochemistry-Paraffin: beta 2-Microglobulin Antibody (B2M/1118) [NBP2-44467]](https://resources.bio-techne.com/images/products/beta-2-Microglobulin-Antibody-B2M-1118-Immunohistochemistry-Paraffin-NBP2-44467-img0003.jpg "Immunohistochemistry-Paraffin: beta 2-Microglobulin Antibody (B2M/1118) [NBP2-44467]")
Immunohistochemistry-Paraffin: beta 2-Microglobulin Antibody (B2M/1118) [NBP2-44467]
Immunohistochemistry-Paraffin: beta 2-Microglobulin Antibody (B2M/1118) [NBP2-44467] - Human Endometrial Carcinoma stained with Beta-2-Microglobulin Monoclonal Antibody (B2M/1118)![Immunohistochemistry-Paraffin: beta 2-Microglobulin Antibody (B2M/1118) [NBP2-44467]](https://resources.bio-techne.com/images/products/beta-2-Microglobulin-Antibody-B2M-1118-Immunohistochemistry-Paraffin-NBP2-44467-img0001.jpg "Immunohistochemistry-Paraffin: beta 2-Microglobulin Antibody (B2M/1118) [NBP2-44467]")
![Immunohistochemistry-Paraffin: beta 2-Microglobulin Antibody (B2M/1118) [NBP2-44467]](https://resources.bio-techne.com/images/products/beta-2-Microglobulin-Antibody-B2M-1118-Immunohistochemistry-Paraffin-NBP2-44467-img0002.jpg "Immunohistochemistry-Paraffin: beta 2-Microglobulin Antibody (B2M/1118) [NBP2-44467]")
Applications for beta 2-Microglobulin Antibody (B2M/1118)
Application
Recommended Usage
Flow Cytometry
1-2 ug/million cells
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Immunofluorescence
0.5 - 1.0 ug/ml
Immunohistochemistry-Paraffin
1-2 ug/ml
Western Blot
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: beta 2-Microglobulin
Additional beta 2-Microglobulin Products
Product Documents for beta 2-Microglobulin Antibody (B2M/1118)
Product Specific Notices for beta 2-Microglobulin Antibody (B2M/1118)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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