beta 2-Microglobulin Antibody (SPM617) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-47703
![Western Blot: beta 2-Microglobulin Antibody (SPM617)Azide and BSA Free [NBP2-47703]](https://resources.bio-techne.com/images/products/beta-2-Microglobulin-Antibody-SPM617-Azide-and-BSA-Free-Western-Blot-NBP2-47703-img0004.jpg "Western Blot: beta 2-Microglobulin Antibody (SPM617)Azide and BSA Free [NBP2-47703]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Primate
Applications
CyTOF-ready, Flow (Cell Surface), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # SPM617
Format
Azide and BSA Free
Concentration
1.0 mg/ml
Product Summary for beta 2-Microglobulin Antibody (SPM617) - Azide and BSA Free
Immunogen
Full length recombinant human beta 2-Microglobulin protein (Uniprot: P61769)
Reactivity Notes
Non-Human primates.
Localization
Cell Surface and Extracellular (Secreted)
Marker
Renal Failure & Tumor Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Theoretical MW
12 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-44468).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for beta 2-Microglobulin Antibody (SPM617) - Azide and BSA Free
Western Blot: beta 2-Microglobulin Antibody (SPM617)Azide and BSA Free [NBP2-47703]
Western Blot: beta 2-Microglobulin Antibody (SPM617) - Azide and BSA Free [NBP2-47703] - Western Blot Analysis of THP-1 and Raji Cell lysate using beta 2-Microglobulin antibody (SPM617).![Immunohistochemistry-Paraffin: beta 2-Microglobulin Antibody (SPM617) - Azide and BSA Free [NBP2-47703]](https://resources.bio-techne.com/images/products/beta-2-Microglobulin-Antibody-SPM617-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-47703-img0002.jpg "Immunohistochemistry-Paraffin: beta 2-Microglobulin Antibody (SPM617) - Azide and BSA Free [NBP2-47703]")
Immunohistochemistry-Paraffin: beta 2-Microglobulin Antibody (SPM617) - Azide and BSA Free [NBP2-47703]
Immunohistochemistry-Paraffin: beta 2-Microglobulin Antibody (SPM617) - Azide and BSA Free [NBP2-47703] - Human Cervical Carcinoma stained with Beta-2-Microglobulin Monoclonal Antibody (SPM617)![Flow Cytometry: beta 2-Microglobulin Antibody (SPM617) - Azide and BSA Free [NBP2-47703]](https://resources.bio-techne.com/images/products/beta-2-Microglobulin-Antibody-SPM617-Azide-and-BSA-Free-Flow-Cytometry-NBP2-47703-img0003.jpg "Flow Cytometry: beta 2-Microglobulin Antibody (SPM617) - Azide and BSA Free [NBP2-47703]")
Flow Cytometry: beta 2-Microglobulin Antibody (SPM617) - Azide and BSA Free [NBP2-47703]
Flow Cytometry: beta 2-Microglobulin Antibody (SPM617) - Azide and BSA Free [NBP2-47703] - Flow Cytometric Analysis of PFA fixed HeLa cells using beta 2-Microglobulin MAb (SPM617) followed by Goat anti-mouse IgG-CF488 (Blue); Goat anti-mouse IgG-CF488 Is Control (Red).Applications for beta 2-Microglobulin Antibody (SPM617) - Azide and BSA Free
Application
Recommended Usage
Flow (Cell Surface)
1:10-1:1000
Flow Cytometry
0.5 - 1 ug/million cells in 0.1 ml
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Immunofluorescence
0.5 - 1.0 ug/ml
Immunohistochemistry-Paraffin
0.5 - 1.0 ug/ml
Western Blot
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: beta 2-Microglobulin
Alternate Names
B2M, beta 2Microglobulin
Gene Symbol
B2M
Additional beta 2-Microglobulin Products
Product Documents for beta 2-Microglobulin Antibody (SPM617) - Azide and BSA Free
Product Specific Notices for beta 2-Microglobulin Antibody (SPM617) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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