beta 2-Microglobulin Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-87482

Novus Biologicals, part of Bio-Techne
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NBP1-87482
NBP1-87482-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Cited:

Rat

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all beta 2-Microglobulin Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: QRTPKIQVYSRHPAENGKSNFLNCYVSGFHPSDIEVDLLKNGERIEKVEHSDLSFSKDWSFYLLYYTEFTPTEKDEYACRVNHVTLSQPKIVKWDRDM

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for beta 2-Microglobulin Antibody

Immunohistochemistry-Paraffin: beta 2-Microglobulin Antibody [NBP1-87482]

Immunohistochemistry-Paraffin: beta 2-Microglobulin Antibody [NBP1-87482]

Immunohistochemistry-Paraffin: beta 2-Microglobulin Antibody [NBP1-87482] - Staining in human lymph node and skeletal muscle tissues using anti-B2M antibody. Corresponding B2M RNA-seq data are presented for the same tissues.
Western Blot: beta 2-Microglobulin Antibody [NBP1-87482]

Western Blot: beta 2-Microglobulin Antibody [NBP1-87482]

Western Blot: beta 2-Microglobulin Antibody [NBP1-87482] - Analysis in human cell lines HeLa and HEK293. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-HSP90B1.
Immunocytochemistry/ Immunofluorescence: beta 2-Microglobulin Antibody [NBP1-87482]

Immunocytochemistry/ Immunofluorescence: beta 2-Microglobulin Antibody [NBP1-87482]

Immunocytochemistry/Immunofluorescence: beta 2-Microglobulin Antibody [NBP1-87482] - Staining of human cell line U-251 MG shows localization to plasma membrane, cytosol and the Golgi apparatus. Antibody staining is shown in green.
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Applications for beta 2-Microglobulin Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: beta 2-Microglobulin

Beta-2-microglobulin is a serum protein found in association with the major histocompatibility complex (MHC) class I heavy chain on the surface of nearly all nucleated cells (Gussow et al., 1987 [PubMed 3312414]).[supplied by OMIM]

Alternate Names

B2M, beta 2Microglobulin

Gene Symbol

B2M

Additional beta 2-Microglobulin Products

Product Documents for beta 2-Microglobulin Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for beta 2-Microglobulin Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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