beta 2-Microglobulin Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-15566

Novus Biologicals, part of Bio-Techne
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NBP2-15566
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Monkey

Predicted:

Chimpanzee (100%), Rhesus Macaque (92%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all beta 2-Microglobulin Antibodies »

Product Summary for beta 2-Microglobulin Antibody

Immunogen

Full length human beta 2-Microglobulin Recombinant protein.

Reactivity Notes

Marmoset (100%).

Localization

Secreted

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

14 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for beta 2-Microglobulin Antibody

Western Blot: beta 2-Microglobulin Antibody [NBP2-15566]

Western Blot: beta 2-Microglobulin Antibody [NBP2-15566]

Western Blot: beta 2-Microglobulin Antibody [NBP2-15566] - Lane 1: HEK cells. Lane 2: HEK cells with siRNA against beta 2-Microglobulin antibody. Lane 3: HEK cells stimulated for increased beta 2-Microglobulin expression. Image from verified customer review.
Immunocytochemistry/ Immunofluorescence: beta 2-Microglobulin Antibody [NBP2-15566]

Immunocytochemistry/ Immunofluorescence: beta 2-Microglobulin Antibody [NBP2-15566]

Immunocytochemistry/Immunofluorescence: beta 2-Microglobulin Antibody [NBP2-15566] - beta 2-Microglobulin antibody detects beta 2 Microglobulin protein by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: beta 2-Microglobulin stained by beta 2-Microglobulin antibody diluted at 1:500. Blue: Fluoroshield with DAPI.
Immunohistochemistry-Paraffin: beta 2-Microglobulin Antibody [NBP2-15566]

Immunohistochemistry-Paraffin: beta 2-Microglobulin Antibody [NBP2-15566]

Immunohistochemistry-Paraffin: beta 2-Microglobulin Antibody [NBP2-15566] - Mouse spleen. B2M stained by Beta 2 Microglobulin antibody diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
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Applications for beta 2-Microglobulin Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Assay dependent

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Immunoprecipitation

1:100-1:500

Western Blot

1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using NBP2-15566 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: beta 2-Microglobulin

Beta-2-microglobulin is a serum protein found in association with the major histocompatibility complex (MHC) class I heavy chain on the surface of nearly all nucleated cells (Gussow et al., 1987 [PubMed 3312414]).[supplied by OMIM]

Alternate Names

B2M, beta 2Microglobulin

Gene Symbol

B2M

UniProt

P61769

Additional beta 2-Microglobulin Products

Product Documents for beta 2-Microglobulin Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for beta 2-Microglobulin Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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