beta-Actin Antibody (AC-15) [Biotin]
Novus Biologicals, part of Bio-Techne | Catalog # NB110-67828B
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human, Mouse, Rat, Porcine, Bovine, Canine, Chicken, Drosophila (Negative), Feline, Fish, Guinea Pig, Hamster, Leech, Mammal, Primate, Rabbit, Sheep, Squirrel, Turkey, Zebrafish
Applications
Chromatin Immunoprecipitation (ChIP), Flow Cytometry
Label
Biotin
Antibody Source
Monoclonal Mouse IgG1 Clone # AC-15
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This beta-Actin Antibody (AC-15) was made to a slightly modified Beta-cytoplasmic actin N-terminal peptide, Ac-Asp-Asp-Asp-Ile-Ala-Ala-Leu-Val-Ile-Asp-Asn-Gly-Ser-Gly-Lys, conjugated to KLH.
Reactivity Notes
The antibody reacts with b-actin-expressing cells in bovine, canine, chicken, feline, carp, leech, guinea pig, human, mouse, porcine, rabbit, rat, and sheep. Does not react with amoeba or drosophila. Does not cross react with adult cardiac or skeletal muscle or amoeba beta actin. Not yet tested in other species. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.
Specificity
Beta-actin (AC-15). In staining of chicken gizzard ultrathin tissue cryosections, the antibody labels the dense bodies and longitudinal channels linking consecutive dense bodies that are also occupied by desmin and the membrane-associated dense plaque. It does not stain adult cardiac and skeletal muscles except for traces due to contaminations of the sample with non-muscle cells, or if embryonic tissue is being used.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Applications for beta-Actin Antibody (AC-15) [Biotin]
Application
Recommended Usage
Chromatin Immunoprecipitation (ChIP)
Optimal dilutions of this antibody should be experimentally determined.
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
PBS
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: beta-Actin
References
1. Vandekerckhove J, Weber K. 1978. At least six different actins are expressed in a higher mammal: an analysis based on the amino acid sequence of the amino-terminal tryptic peptide. J Mol Biol. 126(4):783-802. PMID: 745245
2. Gimona M, Vandekerckhove J, Goethals M, Herzog M, Lando Z, Small JV. (1994) Beta-actin specific monoclonal antibody. Cell Motil Cytoskeleton. 27(2):108-16. PMID: 8162619
3. Holden VI, Lenio S, Kuick R, Ramakrishnan SK, Shah YM, Bachman MA. (2014) Bacterial siderophores that evade or overwhelm Lipocalin 2 induce HIF-1a and pro-inflammatory cytokine secretion in cultured respiratory epithelial cells Infect Immun. 82(9):3826-36 PMID: 24980968
4. Tsai WL, Yeh PH, Tsai CY, Ting CT, Chiu YH, Tao MH, Li WC, Hung SC. (2016) Efficient Programming of Human Mesenchymal Stem Cell Derived Hepatocytes by Epigenetic Regulations. J. Gastroenterol. Hepatol. 32(1):261-269. PMID: 27218433
Alternate Names
ACTB, betaActin
Gene Symbol
ACTB
Additional beta-Actin Products
Product Documents for beta-Actin Antibody (AC-15) [Biotin]
Product Specific Notices for beta-Actin Antibody (AC-15) [Biotin]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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