beta-Actin Antibody (M008)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-23324
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # M008
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Clone (M008) was generated from a proprietary antigen related to human beta-Actin in MDA-MB-231 breast cancer cell line. [UniProt# P60709]
Specificity
Clone M008 detects a 42 kDa* protein corresponding to the molecular mass of beta-actin on SDS-PAGE immunoblots of human cancer cell lines, as well as human recombinant beta actin. This actin antibody preferentially detects human beta-actin with only weak reactivity toward actins in rat, mouse, or rabbit.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Theoretical MW
42 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Stable for at least 1 year at -20°C.
Scientific Data Images for beta-Actin Antibody (M008)
Western Blot: beta-Actin Antibody (M008) [NBP3-23324] -
Western Blot: beta-Actin Antibody (M008) [NBP3-23324] - Western blot analysis of human HUVEC-CS (lane 1), rabbit spleen fibroblast (lane 2), human Jurkat (lane 3), human LNCaP (lane 4), human HeLa (lane 5), and mouse F9 (lane 6) cell lysates. The blot was probed with mouse monoclonal anti-beta-Actin (NBP3-23324) at 1:1000 (lanes 1-6).
Immunocytochemistry/Immunofluorescence: beta-Actin Antibody (M008) [NBP3-23324] -
Immunocytochemistry/Immunofluorescence: beta-Actin Antibody (M008) [NBP3-23324] - Immunocytochemical labeling of beta-Actin in paraformaldehyde fixed human MeWo cells. The cells were labeled with mouse monoclonal anti-beta-Actin (clone M008). The antibody was detected using goat anti-mouse DyLight (R) 594.Applications for beta-Actin Antibody (M008)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50
Immunoprecipitation
1:100
Western Blot
1:1000
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS, 1 mg/ml BSA and 50% glycerol
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C.
Background: beta-Actin
References
1. Vandekerckhove J, Weber K. 1978. At least six different actins are expressed in a higher mammal: an analysis based on the amino acid sequence of the amino-terminal tryptic peptide. J Mol Biol. 126(4):783-802. PMID: 745245
2. Gimona M, Vandekerckhove J, Goethals M, Herzog M, Lando Z, Small JV. (1994) Beta-actin specific monoclonal antibody. Cell Motil Cytoskeleton. 27(2):108-16. PMID: 8162619
3. Holden VI, Lenio S, Kuick R, Ramakrishnan SK, Shah YM, Bachman MA. (2014) Bacterial siderophores that evade or overwhelm Lipocalin 2 induce HIF-1a and pro-inflammatory cytokine secretion in cultured respiratory epithelial cells Infect Immun. 82(9):3826-36 PMID: 24980968
4. Tsai WL, Yeh PH, Tsai CY, Ting CT, Chiu YH, Tao MH, Li WC, Hung SC. (2016) Efficient Programming of Human Mesenchymal Stem Cell Derived Hepatocytes by Epigenetic Regulations. J. Gastroenterol. Hepatol. 32(1):261-269. PMID: 27218433
Alternate Names
ACTB, betaActin
Gene Symbol
ACTB
Additional beta-Actin Products
Product Documents for beta-Actin Antibody (M008)
Product Specific Notices for beta-Actin Antibody (M008)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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