beta-Actin Antibody

Western Blot: beta-Actin Antibody [NB600-505]

Western Blot: beta-Actin Antibody [NB600-505] - analysis of Beta Actin on HeLa and LNCaP cell lysates(25ug/lane) using anti-Beta Actin antibody. Image from verified customer review.

Western Blot: beta-Actin Antibody [NB600-505]

Western Blot: beta-Actin Antibody [NB600-505] - Lane 1: molecular weight. Lane 2: human embryonic kidney 293. Lane 3: human lung carcinoma A549. Lane 4: mouse brain. Load: 35 ug per lane. Primary antibody: Beta Actin antibody at 1:1,500 for overnight at 4C. Secondary antibody: IRDye800 rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 42 kDa corresponding to beta Actin (arrowhead). Other band(s): none.

beta-Actin Antibody

Immunohistochemistry of Rabbit beta-Actin Antibody. Tissue: sections 4.2 mm thick of rana pipiens tissue. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Beta Actin antibody at 1:200 for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: Beta Actin is at the neuromuscular junction. Staining: Beta Actin as precipitated green signal with red and blue counterstain.

Western Blot: beta-Actin Antibody [NB600-505] -

(A) Representative western blot bands: MMP-9, MMP-12 and beta-actin in lung homogenates of the CS and Air groups from different gels. Full-length gels and blots are shown in a supplementary information (Supplemental Fig. 2) (B,C) Expression of MMP-9 (B) and MMP-12 (C) in the CS group compared with the Air group after normalization to beta-actin. (D) Total cells per 1 ml bronchoalveolar lavage fluid. (E) The ratio of neutrophil in bronchoalveolar lavage fluid. *p < 0.05 vs corresponding Air groups.

Western Blot: beta-Actin Antibody [NB600-505] -

Western Blot: beta-Actin Antibody [NB600-505] - SUM149PT cells express a truncated partially functional BRCA1 protein.Cells were irradiated with 6 Gy & lysed after 6 hours for analysis by Western blotting. Data for three independent experiments are shown (Exp 1–3). Note the band shifting after irradiation probably reflecting changes in the phosphorylation status. Mr, molecular weight marker; BRCA1-FL, full-length BRCA1 protein; BRCA1-delta Ex11, truncated BRCA1 protein lacking exon 11. Image collected & cropped by CiteAb from the following publication (https://www.nature.com/articles/srep28217), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: beta-Actin Antibody [NB600-505] -

Western Blot: beta-Actin Antibody [NB600-505] - BRCA1-mutant cell lines show distinct sensitivities to 6-thoguanine.(a) Sensitivity of BRCA1-mutant (red) & BRCA1-wild-type (blue) cell lines to 6-thioguanine was tested in 96-well plates in four replicas using CellTiter-Blue metabolic assay. Each data point represents an average of four replicas. Non-linear regression curves were calculated using GraphPad Prism. (b) Western blot demonstrating that BRCA1-mutant (red) cell lines express full-length 53BP1 protein, but not BRCA1. (c) Quantification of 53BP1 protein expression for select cell lines. Individual normalized band intensity values in arbitrary units (A.U.) from three independent experiments are shown. Red lines show average values for each cell line. Statistical significance was evaluated with the Dunn’s multiple comparisons test following a significant non-parametric Kruskal-Wallis test using GraphPad Prism software. *p < 0,05. (d) Western blot reveals expression of mismatch repair proteins MSH2 & MLH1 in all cells, but lack of the HPRT protein in SUM1315MO2 cell line, likely explaining its resistance to 6-thioguanine. beta-actin is used as loading control. (e) Quantification of HPRT protein expression for select cell lines as described in (c). Image collected & cropped by CiteAb from the following publication (https://www.nature.com/articles/srep28217), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: beta-Actin Antibody [NB600-505] -

Western Blot: beta-Actin Antibody [NB600-505] - BRCA1-mutant cell lines show distinct sensitivities to 6-thoguanine.(a) Sensitivity of BRCA1-mutant (red) & BRCA1-wild-type (blue) cell lines to 6-thioguanine was tested in 96-well plates in four replicas using CellTiter-Blue metabolic assay. Each data point represents an average of four replicas. Non-linear regression curves were calculated using GraphPad Prism. (b) Western blot demonstrating that BRCA1-mutant (red) cell lines express full-length 53BP1 protein, but not BRCA1. (c) Quantification of 53BP1 protein expression for select cell lines. Individual normalized band intensity values in arbitrary units (A.U.) from three independent experiments are shown. Red lines show average values for each cell line. Statistical significance was evaluated with the Dunn’s multiple comparisons test following a significant non-parametric Kruskal-Wallis test using GraphPad Prism software. *p < 0,05. (d) Western blot reveals expression of mismatch repair proteins MSH2 & MLH1 in all cells, but lack of the HPRT protein in SUM1315MO2 cell line, likely explaining its resistance to 6-thioguanine. beta-actin is used as loading control. (e) Quantification of HPRT protein expression for select cell lines as described in (c). Image collected & cropped by CiteAb from the following publication (https://www.nature.com/articles/srep28217), licensed under a CC-BY license. Not internally tested by Novus Biologicals.