beta Amyloid Antibody (MOAB-2) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-13075
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Bacteria, Monkey
Cited:
Human, Mouse, Rat, Bacteria, Primate - Macaca mulatta (Rhesus Macaque)
Applications
Validated:
Dot Blot, ELISA, Immunoblotting, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry Free-Floating, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Cited:
Cytometric Bead Assay Standard, ELISA, IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry Free-Floating, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # MOAB-2
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
This beta Amyloid antibody was developed against recombinant human beta Amyloid 42.
Epitope
Residues 1-4 of beta Amyloid
Reactivity Notes
Use in Mouse reported in scientific literature (PMID:35401818). Monkey reactivity reported in scientific literature (PMID: 29241829). Use in Bacteria reported in scientific literature (PMID:32413239).
Specificity
Beta Amyloid antibody (MOAB-2) recognizes unaggregated, oligomeric and fibrillar forms of beta Amyloid 42 and unaggregated beta Amyloid 40. Does not detect APP.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Theoretical MW
5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for beta Amyloid Antibody (MOAB-2) - BSA Free
Immunohistochemical Staining of beta Amyloid in Normal and 5xFAD Mouse Brain
IHC analysis of beta Amyloid on normal mouse brain (left) and 5xFAD mouse brain (right) using DAB with hematoxylin counterstain. The MOAB-2 antibody was used at 1:20 on normal mouse brain and at 1:400 on 5xFAD mouse brain.Western Blot Detection of beta Amyloid in Mouse Brain Homogenate
Analysis of beta Amyloid (MOAB-2) antibody in (1) 100 pmole beta Amyloid 42, (2) 5xFAD mouse brain homogenate Repetition 1 and (3) 5xFAD mouse brain homogenate Repetition 2.Western Blot Analysis of HEK-APP SWE/BACE1 Cell Lysates Using beta Amyloid Antibody
Western blot analysis in cell lysates from HEK-APP SWE/BACE1 cells probed with an antibody against the C-terminus of APP (Lanes 1 and 2) and beta Amyloid (MOAB-2, Lanes 3 and 4). Beta Amyloid (MOAB-2) does not detect APP (from PMID: 22423893).Applications for beta Amyloid Antibody (MOAB-2) - BSA Free
Application
Recommended Usage
Dot Blot
reported in scientific literature (PMID 22423893)
ELISA
1:100-1:1000
Immunoblotting
reported in scientific literature (PMID 28314768)
Immunocytochemistry/ Immunofluorescence
1:200-1:500
Immunohistochemistry
1:200-1:1000
Immunohistochemistry Free-Floating
reported in scientific literature (PMID 25747037)
Immunohistochemistry-Paraffin
1:200-1:1000
Immunoprecipitation
1:200-1:1000
Western Blot
1:1000-1:5000
Application Notes
In Western blot, a band can be seen at ~4 kDa, representing the beta Amyloid monomer. Larger bands may also be seen representing the unaggregated, oligomeric, and fibrillar forms of beta Amyloid. For higher beta Amyloid yield in WB, please follow the extraction protocol described in Youmans et al, J Neurosci Methods. 2011 March 15; 196(1): 51-59 (PMID: 21219931).
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 2 reviews rated 4.5 using NBP2-13075 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Format
BSA Free
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Amyloid beta
Pyroglutamate amyloid beta peptides (pGlu-Abeta) are N-terminal truncations in which the N-terminal glutamate is cyclized to pyroglutamate resulting in pGlu-Abeta (3-40/42 and 11-40/42) (4). This pyrE modification may have a greater propensity to aggregate under physiological conditions and has been implicated as the molecular species responsible for seeding larger oligomers of amyloid beta.
References
1. Chen GF, Xu TH, Yan Y, Zhou YR, Jiang Y, Melcher K, Xu HE. (2017) Amyloid beta: structure, biology and structure-based therapeutic development. Acta Pharmacol Sin. 38(9):1205-1235. PMID: 28713158
2. De-Paula VJ1, Radanovic M, Diniz BS, Forlenza OV. (2012) Alzheimer's disease. Subcell Biochem. 65:329-52. PMID: 23225010
3. Schaich CL, Maurer MS, Nadkarni NK. (2019) Amyloidosis of the Brain and Heart: Two Sides of the Same Coin? JACC Heart Fail. 7(2):129-131. PMID: 30704604
4. He W, Barrow CJ. (1999) The A beta 3-pyroglutamyl and 11-pyroglutamyl peptides found in senile plaque have greater beta-sheet forming and aggregation propensities in vitro than full-length A beta. Biochemistry. 38(33):10871-7. PMID: 10451383
Additional Amyloid beta Products
Product Documents for beta Amyloid Antibody (MOAB-2) - BSA Free
Product Specific Notices for beta Amyloid Antibody (MOAB-2) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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