beta-Defensin 3 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB200-117
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Rat
Cited:
Human, Rat
Predicted:
Canine (90%), Porcine (90%). Backed by our 100% Guarantee.
Applications
Validated:
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot
Cited:
IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
A synthetic peptide made to an internal portion of Defensin beta 3 (between amino acids 10-60) [UniProt P81534]
Reactivity Notes
Human and rat reactivity reported in (PMID: 21369366).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
7.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for beta-Defensin 3 Antibody - BSA Free
Immunohistochemistry-Paraffin: beta-Defensin 3 Antibody - BSA Free [NB200-117]
Immunohistochemistry-Paraffin: beta-Defensin 3 Antibody [NB200-117] - Distribution of hBD-2 and hBD-3 in normal oral epithelium. Formalin fixed and parafilm embedded normal human oral tissue was labeled with an anti-hBD-2 specific primary antibody, and detected with an AF488 conjugated secondary antibody. hBD-3 was labeled with a rabbit anti-hBD-3 (Novus Biologicals Inc., Littleton, CO, USA), specific primary antibody and detected using an AF594 (purple) conjugated donkey anti-rabbit (Invitrogen). Note green fluorescence detection of hBD-2 localized to the stratum spinosum and stratum granulosum, while pink/red fluorescence detects hBD-3 exclusively to the stratum basale. hBD-1, not shown, is localized to the same regions as hBD-2. Image collected and cropped by CiteAb from the following publication (https://journal.frontiersin.org/article/10.3389/fimmu.2012.00294/abstract) licensed under a CC-BY license.Immunohistochemistry-Paraffin: beta-Defensin 3 Antibody - BSA Free [NB200-117]
Immunohistochemistry-Paraffin: beta-Defensin 3 Antibody [NB200-117] - Analysis of beta-Defensin 3 in human skin.Immunohistochemistry: beta-Defensin 3 Antibody - BSA Free [NB200-117] -
Immunohistochemistry: beta-Defensin 3 Antibody - BSA Free [NB200-117] - Topical application of PALA enhances AMP production in human skin explants. (a) HBD2 protein levels are increased in tissue homogenates of infected human skin explants by PALA or Neosporin treatment. Human skin biopsies were infected with MRSA for 1 h & then treated with the indicated ointments for 24 hours in triplicate. HBD2 levels were measured in tissue homogenates by ELISA (n = 3–4 donors). Mean ± SD; significance determined by 1-way ANOVA & Bonferroni multiple comparison test; *p < 0.05. (b) Immunohistochemistry staining of HBD2 in MRSA infected human skin explants described in (a). Representative image of 3 donors. (c) HBD3 protein levels in tissue homogenates of infected human skin explants are unaffected by PALA or Neosporin treatment. HBD3 levels were measured in tissue homogenates described in (a) by ELISA (n = 4 donors). Mean ± SD; significance determined by 1-way ANOVA & Bonferroni multiple comparison test; p > 0.05. (d) Immunohistochemistry staining of HBD3 in MRSA infected human skin explants described in (a). Representative image of 4 donors. (e) LL-37 protein levels are increased in tissue homogenates of infected human skin explants by PALA treatment. LL-37 levels were measured in tissue homogenates described in (a) by ELISA (n = 4–5 donors). Mean ± SD; significance determined by 1-way ANOVA & Bonferroni multiple comparison test; *p < 0.05. (f) Immunohistochemistry staining of LL-37 in MRSA infected human skin explants described in (a). Representative image of 6 donors. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/29880914), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Applications for beta-Defensin 3 Antibody - BSA Free
Application
Recommended Usage
ELISA
1:100-1:2000
Immunocytochemistry/ Immunofluorescence
reported in scientific literature
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Frozen
1:10-1:500
Immunohistochemistry-Paraffin
1:10-1:500
Western Blot
1:500-1:1000
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: beta-Defensin 3
Alternate Names
BD14, BD3, betaDefensin 3, DEFB103A, DEFB103B, DEFB3, HBD-3
Entrez Gene IDs
55894 (Human)
Gene Symbol
DEFB103B
UniProt
Additional beta-Defensin 3 Products
Product Documents for beta-Defensin 3 Antibody - BSA Free
Product Specific Notices for beta-Defensin 3 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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