Beta Hydroxysteroid Dehydrogenase Antibody (1E8)
Novus Biologicals, part of Bio-Techne | Catalog # H00003284-M02
Key Product Details
Species Reactivity
Human
Applications
ELISA, Sandwich ELISA, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 1E8
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
HSD3B2 (NP_000189.1, 33 a.a. ~ 122 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. ALDKAFRPELREEFSKLQNRTKLTVLEGDILDEPFLKRACQDVSVVIHTACIIDVFGVTHRESIMNVNVKGTQLLLEACVQASVPVFIYT
Specificity
HSD3B2 - hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 2 (1E8)
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Scientific Data Images for Beta Hydroxysteroid Dehydrogenase Antibody (1E8)
![Western Blot: Beta Hydroxysteroid Dehydrogenase Antibody (1E8) [H00003284-M02]](https://resources.bio-techne.com/images/products/Beta-Hydroxysteroid-Dehydrogenase-Antibody-1E8-Western-Blot-H00003284-M02-img0002.jpg "Western Blot: Beta Hydroxysteroid Dehydrogenase Antibody (1E8) [H00003284-M02]")
Western Blot: Beta Hydroxysteroid Dehydrogenase Antibody (1E8) [H00003284-M02]
Western Blot: Beta Hydroxysteroid Dehydrogenase Antibody (1E8) [H00003284-M02] - Analysis of HSD3B2 expression in transfected 293T cell line by HSD3B2 monoclonal antibody (M02), clone 1E8.Lane 1: HSD3B2 transfected lysate(42.1 KDa).Lane 2: Non-transfected lysate.
Sandwich ELISA: Beta Hydroxysteroid Dehydrogenase Antibody (1E8) [H00003284-M02] - Detection limit for recombinant GST tagged HSD3B2 is 0.3 ng/ml as a capture antibody.
Applications for Beta Hydroxysteroid Dehydrogenase Antibody (1E8)
Application
Recommended Usage
Western Blot
1:500
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: Beta Hydroxysteroid Dehydrogenase
Alternate Names
3 beta-HSD type II, 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 2, 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type II, 3-beta-HSD II, delta 5-delta 4-isomerase type II, HSD3B, HSDB, HSDB3B, hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 2,3-beta-hydroxy-Delta(5)-steroid dehydrogenase, progesterone reductase, SDR11E2, short chain dehydrogenase/reductase family 11E, member 2,3-beta-hydroxy-5-ene steroid dehydrogenase
Entrez Gene IDs
3284 (Human)
Gene Symbol
HSD3B2
UniProt
Additional Beta Hydroxysteroid Dehydrogenase Products
Product Documents for Beta Hydroxysteroid Dehydrogenase Antibody (1E8)
Product Specific Notices for Beta Hydroxysteroid Dehydrogenase Antibody (1E8)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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