beta I Tubulin Antibody (2Z6P1)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15928
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Bovine, Canine, Monkey
Applications
Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 2Z6P1
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for beta I Tubulin Antibody (2Z6P1)
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 352-451 of human beta I Tubulin (Q9H4B7). AVCDIPPRGLSMAATFIGNNTAIQEIFNRVSEHFSAMFKRKAFVHWYTSEGMDINEFGEAENNIHDLVSEYQQFQDAKAVLEEDEEVTEEAEMEPEDKGH
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for beta I Tubulin Antibody (2Z6P1)
Western Blot: beta I Tubulin Antibody (2Z6P1) [NBP3-15928]
Western Blot: beta I Tubulin Antibody (1C3J0) [NBP3-15928] - Western blot analysis of extracts of various cell lines, using beta I Tubulin Rabbit mAb (NBP3-15928) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.Immunocytochemistry/ Immunofluorescence: beta I Tubulin Antibody (2Z6P1) [NBP3-15928]
Immunocytochemistry/Immunofluorescence: beta I Tubulin Antibody (1C3J0) [NBP3-15928] - Immunofluorescence analysis of U-2 OS cells using beta I Tubulin Rabbit mAb (NBP3-15928) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.Immunocytochemistry/ Immunofluorescence: beta I Tubulin Antibody (2Z6P1) [NBP3-15928]
Immunocytochemistry/Immunofluorescence: beta I Tubulin Antibody (1C3J0) [NBP3-15928] - Immunofluorescence analysis of C6 cells using beta I Tubulin Rabbit mAb (NBP3-15928) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.Applications for beta I Tubulin Antibody (2Z6P1)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50 - 1:200
Western Blot
1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 0.05% BSA, 50% glycerol, pH7.3
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: beta-I Tubulin
Long Name
Tubulin, Beta 1 Class VI
Alternate Names
betaI Tubulin, TUBB1
Gene Symbol
TUBB1
Additional beta-I Tubulin Products
Product Documents for beta I Tubulin Antibody (2Z6P1)
Product Specific Notices for beta I Tubulin Antibody (2Z6P1)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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