Key Product Details

Species Reactivity

Human, Mouse, Rat, Canine, Monkey

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI4A3

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all beta-I Tubulin Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human TUBB1(NP_110400) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

50.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for beta I Tubulin Antibody (OTI4A3) - Azide and BSA Free

Western Blot: beta I Tubulin Antibody (OTI4A3)Azide and BSA Free [NBP2-72225]

Western Blot: beta I Tubulin Antibody (OTI4A3)Azide and BSA Free [NBP2-72225]

Western Blot: beta I Tubulin Antibody (OTI4A3) - Azide and BSA Free [NBP2-72225] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY beta 1 Tubulin.
Immunocytochemistry/ Immunofluorescence: beta I Tubulin Antibody (OTI4A3) - Azide and BSA Free [NBP2-72225]

Immunocytochemistry/ Immunofluorescence: beta I Tubulin Antibody (OTI4A3) - Azide and BSA Free [NBP2-72225]

Immunocytochemistry/Immunofluorescence: beta I Tubulin Antibody (OTI4A3) - Azide and BSA Free [NBP2-72225] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY beta I Tubulin.
Immunohistochemistry: beta I Tubulin Antibody (OTI4A3) - Azide and BSA Free [NBP2-72225]

Immunohistochemistry: beta I Tubulin Antibody (OTI4A3) - Azide and BSA Free [NBP2-72225]

Immunohistochemistry: beta I Tubulin Antibody (OTI4A3) - Azide and BSA Free [NBP2-72225] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
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Applications for beta I Tubulin Antibody (OTI4A3) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Western Blot

1:200-2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: beta-I Tubulin

Microtubules are involved in a wide variety of cellular processes, including mitosis, morphogenesis, plateletformation, and mobility of cilia and flagella. Circulating platelets carry a single marginal microtubule coil that iswound in 8 to 12 turns and is responsible for platelet shape. TUBB1 is the major beta-tubulin expressed in plateletsand megakaryocytes and is required for optimal platelet assembly (Wang et al., 1986 (PubMed 3782288); Schulze et al.,2004 (PubMed 15315966)).(supplied by OMIM)

Long Name

Tubulin, Beta 1 Class VI

Alternate Names

betaI Tubulin, TUBB1

Gene Symbol

TUBB1

Additional beta-I Tubulin Products

Product Documents for beta I Tubulin Antibody (OTI4A3) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for beta I Tubulin Antibody (OTI4A3) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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