beta-III Tubulin Antibody (GT1338)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13505

Novus Biologicals, part of Bio-Techne
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NBP3-13505
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (100%), Chimpanzee (100%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # GT1338

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all beta-III Tubulin Antibodies »

Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human beta-III Tubulin. The exact sequence is proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Description

Centrifuge briefly prior to opening.

Scientific Data Images for beta-III Tubulin Antibody (GT1338)

Western Blot: beta-III Tubulin Antibody (GT1338) [NBP3-13505]

Western Blot: beta-III Tubulin Antibody (GT1338) [NBP3-13505]

Western Blot: beta-III Tubulin Antibody (GT1338) [NBP3-13505] - Wild-type (WT) and beta-III Tubulin knockout (KO) HeLa cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with beta-III Tubulin antibody [GT1338] (NBP3-13505) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: beta-III Tubulin Antibody (GT1338) [NBP3-13505]

Immunocytochemistry/ Immunofluorescence: beta-III Tubulin Antibody (GT1338) [NBP3-13505]

Immunocytochemistry/Immunofluorescence: beta-III Tubulin Antibody (GT1338) [NBP3-13505] - beta-III Tubulin antibody [GT1338] detects beta-III Tubulin protein at cytoplasm by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min. Green: beta-III Tubulin protein stained by beta-III Tubulin antibody [GT1338] (NBP3-13505) diluted at 1:500. Red: NeuN, stained by NeuN antibody diluted at 1:1000. Blue: Fluoroshield with DAPI.
Immunohistochemistry-Paraffin: beta-III Tubulin Antibody (GT1338) [NBP3-13505]

Immunohistochemistry-Paraffin: beta-III Tubulin Antibody (GT1338) [NBP3-13505]

Immunohistochemistry-Paraffin: beta-III Tubulin Antibody (GT1338) [NBP3-13505] - beta-III Tubulin antibody [GT1338] detects beta-III Tubulin protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse E10.5 embryo. Red: beta-III Tubulin stained by beta-III Tubulin antibody [GT1338] (NBP3-13505) diluted at 1:500. Blue: Fluoroshield with DAPI.
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Applications for beta-III Tubulin Antibody (GT1338)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: beta-III Tubulin

Beta-III Tubulin is abundant in the central and peripheral nervous systems where it is prominently expressed during fetal and postnatal development. Beta III Tubulin is often referred to as TUJ1 because Tuj-1 is the clone name of the original monoclonal antibody. The Tuj1 clone is such a popular neuronal marker that Tuj-1 is now used as a synonym for beta III tubulin. The distribution of Tubulin Beta 3 is neuron-associated, exhibiting distinct temporospatial gradients according to the regional neuroepithelia of origin. However, transient expression of the Tubulin Beta 3 protein is also present in the subventricular zones of the CNS comprising putative neuronal- and/or glial precursor cells, as well as in Kulchitsky neuroendocrine cells of the fetal respiratory epithelium. This temporally restricted, potentially non-neuronal expression may have implications in the identification of presumptive neurons derived from embryonic stem cells. Mutation on beta-tubulin has been linked to anti-cancer drug resistance in lung cancers.

Alternate Names

beta-Tubulin III, Beta3-tubulin, betaIII Tubulin, Class III beta-tubulin, TUBB3, Tubulin Beta 3, Tubulin beta-4, Tubulin beta-III

Gene Symbol

TUBB3

Additional beta-III Tubulin Products

Product Documents for beta-III Tubulin Antibody (GT1338)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for beta-III Tubulin Antibody (GT1338)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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