beta-III Tubulin Antibody (TU-20) [FITC]

Novus Biologicals, part of Bio-Techne | Catalog # NB500-620

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Mouse, Rat, Canine

Applications

Flow (Intracellular), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

FITC (Excitation = 495 nm, Emission = 519 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # TU-20

Concentration

1.0 mg/ml

View all beta-III Tubulin Antibodies »

Product Specifications

Immunogen

Peptide (C) 441-448 coupled to maleimide-activated keyhole limpet hemocyanin via cysteine added to the N-terminus of the neuron-specific peptide.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions. Rat reactivity reported in scientific literature (PMID: 23984759).

Specificity

The antibody TU-20 recognizes C-terminal peptide sequence ESESQGPK (aa 441-448) of neuron-specific human betaIII-tubulin.

Marker

Neuronal Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for beta-III Tubulin Antibody (TU-20) [FITC]

Immunocytochemistry/Immunofluorescence: beta-III Tubulin Antibody (TU-20) [FITC] [NB500-620] - Immunofluorescence staining of P-19 mouse embryonal carcinoma cell line stimulated to neuronal differentiation by retinoic acid. 2A - Microtubules decorated with neuron-specific anti-betaIII-tubulin (TU-20; red). 2B - Merged image of co-staining with anti-beta-tubulin (TU-06; green). Superposition of red and green colours provided yellow staining. Nuclei were stained with DNA-binding dye (blue).
Immunocytochemistry/Immunofluorescence: beta-III Tubulin Antibody (TU-20) [FITC] [NB500-620] - Immunofluorescence staining of Neuro2a mouse neuroblastoma cell line using anti-betaIII-tubulin (TU-20 FITC, green, 1 ug/ml). Nuclei were stained with DAPI (blue).
Immunocytochemistry/Immunofluorescence: beta-III Tubulin Antibody (TU-20) [FITC] [NB500-620] - Staining of Neuro2a mouse neuroblastoma cell line using anti-betaIII-tubulin (TU-20; green; 3 ug/ml). Nuclei were stained with DAPI (blue).

Applications for beta-III Tubulin Antibody (TU-20) [FITC]

Application
Recommended Usage

Flow (Intracellular)

1-3 ug/ml

Flow Cytometry

1-3 ug/ml

Immunocytochemistry/ Immunofluorescence

1:400

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

10 ug/ml

Western Blot

1-2 ug/ml
Application Notes
For Immunohistochemistry-Paraffin: Use standard ABC technique (DAB+). Purity is > 95% (by SDS-PAGE). Western Blot: reducing conditions

Formulation, Preparation, and Storage

Purification

Size Exclusion Chromatography

Formulation

PBS (pH 7.4)

Preservative

0.9% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: beta-III Tubulin

Tubulin is the major building block of microtubules. This intracellular cylindrical filamentous structure is present in almost all eukaryotic cells. Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement, and the cytoskeleton. Except in the simplest eukaryotes, tubulin exists in all cells as a mixture of similar, but not identical, sets of alpha and beta tubulin polypeptides. Within either set of polypeptides, individual subunits diverge from each other (both within and across species) at less than 10% of the amino acid positions. The most extreme diversity is localized to the 15 residues of the carboxy terminal. For beta tubulin five evolutionarily conserved isotype clones have been identified. These are almost totally conserved in the subunits utilized in the same cell types of different species, with the exception of the hematopoietic beta tubulin which is the most highly divergent in sequence and is not conserved between species. Research has been centered around the hypothesis that these beta tubulin isotypes contribute to unique functional properties. It has been reported that the different isotypes of tubulin differ from each other in their ability to polymerize into microtubules. The monoclonal antibody from hybridoma SDL.3D10 can stimulate microtubule assembly when reconstituted with tubulin, tau or MAP2

Alternate Names

beta-Tubulin III, Beta3-tubulin, betaIII Tubulin, Class III beta-tubulin, TUBB3, Tubulin Beta 3, Tubulin beta-4, Tubulin beta-III

Gene Symbol

TUBB3

Additional beta-III Tubulin Products

Product Documents for beta-III Tubulin Antibody (TU-20) [FITC]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for beta-III Tubulin Antibody (TU-20) [FITC]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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