beta-III Tubulin Antibody (TUBB3/3732)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-07386

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Canine

Applications

Immunohistochemistry-Paraffin, Protein Array, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # TUBB3/3732

Concentration

0.2 mg/ml

View all beta-III Tubulin Antibodies »

Product Summary for beta-III Tubulin Antibody (TUBB3/3732)

Immunogen

A synthetic peptide (aa 437-450) of human Tubulin beta 3 protein (TUBB3); coupled to KLH. (Uniprot: Q13509)

Localization

Cytoplasmic

Marker

Neuronal& Stem Cell Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Theoretical MW

55 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08455)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for beta-III Tubulin Antibody (TUBB3/3732)

Western Blot: beta-III Tubulin Antibody (TUBB3/3732) [NBP3-07386]

Western Blot: beta-III Tubulin Antibody (TUBB3/3732) [NBP3-07386]

Western Blot: beta-III Tubulin Antibody (TUBB3/3732) [NBP3-07386] - Western Blot Analysis of U87 cell lysate using beta-III Tubulin Mouse Monoclonal Antibody (TUBB3/3732).
Immunohistochemistry-Paraffin: beta-III Tubulin Antibody (TUBB3/3732) [NBP3-07386]

Immunohistochemistry-Paraffin: beta-III Tubulin Antibody (TUBB3/3732) [NBP3-07386]

Immunohistochemistry-Paraffin: beta-III Tubulin Antibody (TUBB3/3732) [NBP3-07386] - Formalin-fixed, paraffin-embedded human bladder carcinoma stained with beta-III Tubulin Mouse Monoclonal Antibody (TUBB3/3732).
Protein Array: beta-III Tubulin Antibody (TUBB3/3732) [NBP3-07386]

Protein Array: beta-III Tubulin Antibody (TUBB3/3732) [NBP3-07386]

Protein Array: beta-III Tubulin Antibody (TUBB3/3732) [NBP3-07386] - Analysis of Protein Array containing more than 21,000 full-length human proteins using beta-III Tubulin Mouse Monoclonal Antibody (TUBB3/3732).

Applications for beta-III Tubulin Antibody (TUBB3/3732)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2 ug/ml

Western Blot

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: beta-III Tubulin

Beta-III Tubulin is abundant in the central and peripheral nervous systems where it is prominently expressed during fetal and postnatal development. Beta III Tubulin is often referred to as TUJ1 because Tuj-1 is the clone name of the original monoclonal antibody. The Tuj1 clone is such a popular neuronal marker that Tuj-1 is now used as a synonym for beta III tubulin. The distribution of Tubulin Beta 3 is neuron-associated, exhibiting distinct temporospatial gradients according to the regional neuroepithelia of origin. However, transient expression of the Tubulin Beta 3 protein is also present in the subventricular zones of the CNS comprising putative neuronal- and/or glial precursor cells, as well as in Kulchitsky neuroendocrine cells of the fetal respiratory epithelium. This temporally restricted, potentially non-neuronal expression may have implications in the identification of presumptive neurons derived from embryonic stem cells. Mutation on beta-tubulin has been linked to anti-cancer drug resistance in lung cancers.

Alternate Names

beta-Tubulin III, Beta3-tubulin, betaIII Tubulin, Class III beta-tubulin, TUBB3, Tubulin Beta 3, Tubulin beta-4, Tubulin beta-III

Gene Symbol

TUBB3

UniProt

Q13509 (Human)

Additional beta-III Tubulin Products

Product Documents for beta-III Tubulin Antibody (TUBB3/3732)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for beta-III Tubulin Antibody (TUBB3/3732)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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