beta-III Tubulin Antibody (TUBB3/7090R) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-14268
Recombinant Monoclonal Antibody
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # TUBB3/7090R
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Summary for beta-III Tubulin Antibody (TUBB3/7090R) - Azide and BSA Free
Immunogen
A synthetic peptide (aa 437-450) of human Tubulin beta 3 protein (TUBB3); coupled to KLH. (Uniprot: Q13509 )
Localization
Cytoplasm
Marker
Neuronal & Stem Cell Marker
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
55 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-13837).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Scientific Data Images for beta-III Tubulin Antibody (TUBB3/7090R) - Azide and BSA Free
beta-III Tubulin Antibody (TUBB3/7090R)
Western blot analysis of PC3 cell lysate using beta-III Tubulin antibody (TUBB3/7090R).
beta-III Tubulin Antibody (TUBB3/7090R)
Formalin-fixed, paraffin-embedded human ovarian carcinoma stained with beta-III Tubulin antibody (TUBB3/7090R). HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.Applications for beta-III Tubulin Antibody (TUBB3/7090R) - Azide and BSA Free
Application
Recommended Usage
Immunohistochemistry-Paraffin
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: beta-III Tubulin
Alternate Names
beta-Tubulin III, Beta3-tubulin, betaIII Tubulin, Class III beta-tubulin, TUBB3, Tubulin Beta 3, Tubulin beta-4, Tubulin beta-III
Gene Symbol
TUBB3
Additional beta-III Tubulin Products
Product Documents for beta-III Tubulin Antibody (TUBB3/7090R) - Azide and BSA Free
Product Specific Notices for beta-III Tubulin Antibody (TUBB3/7090R) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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