beta-III Tubulin Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-1612

Novus Biologicals, part of Bio-Techne
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NB100-1612
NB100-1612-0.05ml
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse, Rat

Applications

Validated:

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry Whole-Mount, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot

Cited:

Block/Neutralize, Electron Microscopy, Flow Cytometry, IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry Whole-Mount, In vivo assay, Knockdown Validated, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Chicken IgY

Concentration

0.3 mg/ml

View all beta-III Tubulin Antibodies »

Product Specifications

Immunogen

Chickens were immunized with three synthetic peptide/keyhole limpet hemocyanin (KLH) conjugates. These synthetic peptides corresponded to different regions of beta-III Tubulin, but are shared between the human (NP_AAL28094, NCBI) and rat (AAM28438, NCBI) protein sequences.

Marker

Neuron Cell Marker

Clonality

Polyclonal

Host

Chicken

Isotype

IgY

Theoretical MW

50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for beta-III Tubulin Antibody

Western Blot: beta-III Tubulin Antibody [NB100-1612]

Western Blot: beta-III Tubulin Antibody [NB100-1612]

Western Blot: beta-III Tubulin Antibody [NB100-1612] - The levels of glial fibrillary acidic protein (GFAP) and S100beta as astrocytic protein marker proteins in the hippocampal area. Western blot images using GFAP, tubulin beta-3, and beta-actin antibodies to total protein lysate in hippocampal area. The statistical results of internal standardized GFAP/beta-tubulin ratio by beta-actin levels relative to young adult wild-type C57BL/6J. White and black bars indicate the wild-type C57BL/6J and Tet-mev-1 mice, respectively. Data are expressed as mean +/- SD; *P < 0.05; **P < 0.01; n = >12 in each group. Image collected and cropped by CiteAb from the following publication (https://doi.wiley.com/10.1111/acel.12523), licensed under a CC-BY license.
Immunocytochemistry/ Immunofluorescence: beta-III Tubulin Antibody [NB100-1612]

Immunocytochemistry/ Immunofluorescence: beta-III Tubulin Antibody [NB100-1612]

Immunocytochemistry/Immunofluorescence: beta-III Tubulin Antibody [NB100-1612] - Beta-III Tubulin in rat primary motor neurons. Ab dilution 1:1000 in PBST (0.1% Triton X-100) + 10% GS O/N at 4C. ICC/IF image submitted by a verified customer review.
Western Blot: beta-III Tubulin Antibody [NB100-1612]

Western Blot: beta-III Tubulin Antibody [NB100-1612]

Western Blot: beta-III Tubulin Antibody [NB100-1612] - The levels of glial fibrillary acidic protein (GFAP) and S100beta as astrocytic protein marker proteins in the hippocampal area. Western blot images using S100beta, tubulin beta-3, and beta-actin antibodies to total protein lysate in hippocampal area. The statistical results of internal standardized S100beta/beta-tubulin ratio by beta-actin levels relative to young adult wild-type C57BL/6J. White and black bars indicate the wild-type C57BL/6J and Tet-mev-1 mice, respectively. Data are expressed as mean +/- SD; *P < 0.05; **P < 0.01; n = >12 in each group. Image collected and cropped by CiteAb from the following publication (https://doi.wiley.com/10.1111/acel.12523), licensed under a CC-BY license.
View 2 more images

Applications for beta-III Tubulin Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:2000-1:5000

Immunohistochemistry

1:2000 - 1:5000

Western Blot

1:10000 - 1:20000
Application Notes
Although not tested, this antibody may be useful for immunohistochemistry on frozen sections. Use in KD reported in scientific literature (PMID:33333046). Each of the three antibodies were analyzed by immunohistochemistry (1:2000). using fluorescein-labeled goat anti-chicken IgY (1:500). as the secondary reagent. Use in Immunohistochemistry-Whole mount reported in scientific literature (PMID 27178445). Use in Immunohistochemistry-Paraffin reported in scientific literature (PMID: 27178445). Use in Flow Cytometry reported in scientific literature (PMID: 31755958).

Reviewed Applications

Read 2 reviews rated 4.5 using NB100-1612 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

10mM PBS (0.9% isotonic, w/v, pH 7.2)

Preservative

0.02% Sodium Azide

Concentration

0.3 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: beta-III Tubulin

Beta-III Tubulin is abundant in the central and peripheral nervous systems where it is prominently expressed during fetal and postnatal development. Beta III Tubulin is often referred to as TUJ1 because Tuj-1 is the clone name of the original monoclonal antibody. The Tuj1 clone is such a popular neuronal marker that Tuj-1 is now used as a synonym for beta III tubulin. The distribution of Tubulin Beta 3 is neuron-associated, exhibiting distinct temporospatial gradients according to the regional neuroepithelia of origin. However, transient expression of the Tubulin Beta 3 protein is also present in the subventricular zones of the CNS comprising putative neuronal- and/or glial precursor cells, as well as in Kulchitsky neuroendocrine cells of the fetal respiratory epithelium. This temporally restricted, potentially non-neuronal expression may have implications in the identification of presumptive neurons derived from embryonic stem cells. Mutation on beta-tubulin has been linked to anti-cancer drug resistance in lung cancers.

Alternate Names

beta-Tubulin III, Beta3-tubulin, betaIII Tubulin, Class III beta-tubulin, TUBB3, Tubulin Beta 3, Tubulin beta-4, Tubulin beta-III, TUJ1 antigen, TUJ-1 antigen

Entrez Gene IDs

10381 (Human); 246118 (Rat)

Gene Symbol

TUBB3

UniProt

Q13509 (Human)

Additional beta-III Tubulin Products

Product Documents for beta-III Tubulin Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for beta-III Tubulin Antibody

Chicken products cannot be exported to Canada.

Purification Notes
After repeated injections, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, and the concentrations of the eluates adjusted to 300 ug/ml. Finally, equal volumes of each of the three affinity-purified anti-peptide antibodies were mixed, and the preparation was filter-sterilized.

Storage Notes
Store at 4C in the dark. Under these conditions, the antibodies should have a shelf life of at least 12 months (provided they remain sterile). Do not freeze these antibodies unless you want to store them for longer periods of time. Note, however, that each time an antibody preparation is frozen, about half of its binding activity is lost.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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