beta-Synuclein Antibody (6A10)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-05529

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine, Bovine

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 6A10

Concentration

1 mg/ml

View all beta-Synuclein Antibodies »

Product Summary for beta-Synuclein Antibody (6A10)

Immunogen

C-terminal peptide of human beta-Synuclein EPEGESYEDPPQEEYQEYEPEA coupled to KLH

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

17 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for beta-Synuclein Antibody (6A10)

Western Blot: beta-Synuclein Antibody [NBP3-05529]

Western Blot: beta-Synuclein Antibody [NBP3-05529]

Western Blot: beta-Synuclein Antibody [NBP3-05529] - Western blot analysis of different tissue lysates using beta-Synuclein Antibody at a at a dilution of of 1:1,000 in green: [1] protein standard (red), [2] rat cortex [3] rat cerebellum, [4] mouse cortex, [5] mouse cerebellum, [6] cow cortex, and [7] cow cerebellum. Strong band at about 17 kDa corresponds to beta-Synuclein protein.
Immunocytochemistry/ Immunofluorescence: beta-Synuclein Antibody [NBP3-05529]

Immunocytochemistry/ Immunofluorescence: beta-Synuclein Antibody [NBP3-05529]

Immunocytochemistry/Immunofluorescence: beta-Synuclein Antibody [NBP3-05529] - Immunofluorescent analysis of mouse hippocampus section stained with beta-Synuclein Antibody at a at a dilution of of 1:500 in green, and costained with rabbit polyclonal antibody to C-terminal peptide of rat NF-L at a at a dilution of of 1:5,000, in red. The blue is Hoechst staining of nuclear DNA. Following transcardial perfusion of mouse with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with above antibodies. The beta-Synuclein antibody detects protein concentrated in synaptic regions, while the NF-L antibody labels dendrites and axons of neuronal cells.
Immunocytochemistry/ Immunofluorescence: beta-Synuclein Antibody [NBP3-05529]

Immunocytochemistry/ Immunofluorescence: beta-Synuclein Antibody [NBP3-05529]

Immunocytochemistry/Immunofluorescence: beta-Synuclein Antibody [NBP3-05529] - Immunofluorescent analysis of mouse hippocampus section stained with beta-Synuclein Antibody at a at a dilution of of 1:500 in green, and costained with rabbit polyclonal antibody to C-terminal peptide of rat NF-L at a at a dilution of of 1:5,000, in red. The blue is Hoechst staining of nuclear DNA. Following transcardial perfusion of mouse with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with above antibodies. The beta-Synuclein Antibody detects protein concentrated in synaptic regions, while the NF-L antibody labels dendrites and axons of neuronal cells.

Applications for beta-Synuclein Antibody (6A10)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:500 - 1:1000

Western Blot

1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

50% PBS, 50% glycerol

Preservative

0.035% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Store at -20C long term. Avoid freeze-thaw cycles.

Background: beta-Synuclein

Alpha synuclein is an abundant 140 amino acid neuronal protein, expressed primarily at presynaptic terminals in the central nervous system. Alpha synuclein has been associated with several neurodegenerative diseases. A point mutation in the gene coding for the alpha-synuclein protein was the first discovery linking this protein to a rare familial form of Parkinson's disease (PD). Subsequently, other mutations in the alpha-synuclein gene have been identified in familial PD. The aggregated proteinaceous inclusions called Lewy bodies found in PD and cortical Lewy body dementia (LBD) were discovered to be predominantly alpha-synuclein. Aberrant aggregation of alpha-synuclein has been detected in an increasing number of neurodegenerative diseases, collectively known as synucleopathies. Alpha-synuclein exists physiologically in both soluble and membrane-bound states, in unstructured and alpha-helical conformations, respectively. The physiological function of alpha-synuclein appears to require its translocation between these subcellular compartments and interconversion between the 2 conformations. Abnormal processing of alpha-synuclein is predicted to lead to pathological changes in its binding properties and function (1).

Alternate Names

SNCB, Synuclein-beta

Gene Symbol

SNCB

Additional beta-Synuclein Products

Product Documents for beta-Synuclein Antibody (6A10)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for beta-Synuclein Antibody (6A10)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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