beta Tubulin Antibody (4E4)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-50047

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Monkey

Cited:

Mouse

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 4E4

Concentration

1 mg/ml

View all beta Tubulin Antibodies »

Product Specifications

Immunogen

Pig brain beta Tubulin. [UniProt# Q767L7]

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for beta Tubulin Antibody (4E4)

Western Blot: beta Tubulin Antibody (4E4) [NBP2-50047]

Western Blot: beta Tubulin Antibody (4E4) [NBP2-50047]

Western Blot: beta Tubulin Antibody (4E4) [NBP2-50047] - Analysis of equal amounts of cell line and whole brain lysates using mouse mAb to Beta-tubulin NBP2-50047, dilution 1:5,000 in green: [1] protein standard (red), [2] HEK293, [3] HeLa, [4] SH-SY5Y, [5] COS-1, [6] NIH-3T3, [7] C6 cells, [8] rat brain, and [9] mouse brain. Strong band at 50 kDa corresponds to the Beta-tubulin proteins.
Immunocytochemistry/ Immunofluorescence: beta Tubulin Antibody (4E4) [NBP2-50047]

Immunocytochemistry/ Immunofluorescence: beta Tubulin Antibody (4E4) [NBP2-50047]

Immunocytochemistry/Immunofluorescence: beta Tubulin Antibody (4E4) [NBP2-50047] - HeLa cells stained with NBP2-50047 (green) and chicken antibody to Lamin A+C (NBP2-25152, red) and DNA in blue. NBP2-50047 reveals strong microtubular staining in the cytoplasm of HeLa cells, while the Lamin A+C localizes in the nuclear membrane.

Applications for beta Tubulin Antibody (4E4)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:1000 - 1:5000

Immunohistochemistry

1:1000-1:5000

Western Blot

1:5000 - 1:10000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

50% PBS, 50% glycerol

Preservative

0.035% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: beta Tubulin

Beta Tubulin antibodies are useful as loading controls for Western Blotting and ELISA, as well as in studies invlolving cell structure and division. Tubulin is the major building block of microtubules. This intracellular cylindrical filamentous structure is present in almost all eukaryotic cells. Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement, and in the cytoskeleton.

Long Name

Tubulin beta chain

Alternate Names

OK/SW-cl.56, TUBB, TUBB5

Gene Symbol

TUBB

UniProt

P07437 (Human)

Additional beta Tubulin Products

Product Documents for beta Tubulin Antibody (4E4)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for beta Tubulin Antibody (4E4)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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