beta Tubulin Antibody (4E9G8)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15439
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 4E9G8
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 250-350 of human beta Tubulin (P07437). LRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQVFDAKNMMAACDPRHGRYLTVAAVFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVK
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for beta Tubulin Antibody (4E9G8)
![Immunocytochemistry/ Immunofluorescence: beta Tubulin Antibody (4E9G8) [NBP3-15439]](https://resources.bio-techne.com/images/products/beta-Tubulin-Antibody-4E9G8-Immunocytochemistry-Immunofluorescence-NBP3-15439-img0001.jpg "Immunocytochemistry/ Immunofluorescence: beta Tubulin Antibody (4E9G8) [NBP3-15439]")
Immunocytochemistry/ Immunofluorescence: beta Tubulin Antibody (4E9G8) [NBP3-15439]
Immunocytochemistry/Immunofluorescence: beta Tubulin Antibody (4E9G8) [NBP3-15439] - Immunofluorescence analysis of C6 cells using beta Tubulin antibody (NBP3-15439) at dilution of 1:100. Blue: DAPI for nuclear staining.![Immunocytochemistry/ Immunofluorescence: beta Tubulin Antibody (4E9G8) [NBP3-15439]](https://resources.bio-techne.com/images/products/beta-Tubulin-Antibody-4E9G8-Immunocytochemistry-Immunofluorescence-NBP3-15439-img0002.jpg "Immunocytochemistry/ Immunofluorescence: beta Tubulin Antibody (4E9G8) [NBP3-15439]")
Immunocytochemistry/ Immunofluorescence: beta Tubulin Antibody (4E9G8) [NBP3-15439]
Immunocytochemistry/Immunofluorescence: beta Tubulin Antibody (4E9G8) [NBP3-15439] - Immunofluorescence analysis of NIH/3T3 cells using beta Tubulin antibody (NBP3-15439) at dilution of 1:100. Blue: DAPI for nuclear staining.
Western Blot: beta Tubulin Antibody (4E9G8) [NBP3-15439] -
Western Blot: beta Tubulin Antibody (4E9G8) [NBP3-15439] - Analysis of various lysates using beta-Tubulin Rabbit mAb at 1:5000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 20s.Applications for beta Tubulin Antibody (4E9G8)
Application
Recommended Usage
ELISA
,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Immunocytochemistry/ Immunofluorescence
1:50 - 1:200
Immunohistochemistry
1:50 - 1:200
Immunohistochemistry-Paraffin
1:50 - 1:200
Immunoprecipitation
0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Western Blot
1:5000 - 1:10000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 0.05% BSA, 50% glycerol, pH7.3
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: beta Tubulin
Long Name
Tubulin beta chain
Alternate Names
OK/SW-cl.56, TUBB, TUBB5
Gene Symbol
TUBB
Additional beta Tubulin Products
Product Documents for beta Tubulin Antibody (4E9G8)
Product Specific Notices for beta Tubulin Antibody (4E9G8)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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