beta Tubulin Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-56459
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Bovine, Canine, Chicken, Feline, Monkey, Primate, Xenopus
Cited:
Mouse, Rat, Canine, Feline, Insect - Drosophila
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Simple Western, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
1.0 mg/ml
Product Specifications
Immunogen
A synthetic peptide corresponding to a portion of amino acids 400-450 of human b-tubulin was used as immunogen for this antibody, GenBank no. gi|27227551|gb|AAN85571.1|.
Reactivity Notes
Feline reactivity reported in scientific literature (PMID: 25668772).
Specificity
The amino acid sequence used as immunogen has 100% identity in beta I and beta II tubulin.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for beta Tubulin Antibody
![Western Blot: beta Tubulin Antibody [NB100-56459]](https://resources.bio-techne.com/images/products/beta-Tubulin-Antibody-Western-Blot-NB100-56459-img0004.jpg "Western Blot: beta Tubulin Antibody [NB100-56459]")
Western Blot: beta Tubulin Antibody [NB100-56459]
Western Blot: beta Tubulin Antibody [NB100-56459] - Analysis of beta-Tubulin in A: human brain, B: mouse brain and C: rat brain tissue lysates using the this antibody at 25 ug/mL.![Immunohistochemistry-Paraffin: beta Tubulin Antibody [NB100-56459]](https://resources.bio-techne.com/images/products/beta-Tubulin-Antibody-Immunohistochemistry-Paraffin-NB100-56459-img0005.jpg "Immunohistochemistry-Paraffin: beta Tubulin Antibody [NB100-56459]")
Immunohistochemistry-Paraffin: beta Tubulin Antibody [NB100-56459]
Immunohistochemistry-Paraffin: beta Tubulin Antibody [NB100-56459] - Human kidney tubules stained with beta-Tubulin antibody at 10 ug/mL. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.![Simple Western: beta Tubulin Antibody [NB100-56459]](https://resources.bio-techne.com/images/products/beta-Tubulin-Antibody-Simple-Western-NB100-56459-img0006.jpg "Simple Western: beta Tubulin Antibody [NB100-56459]")
Simple Western: beta Tubulin Antibody [NB100-56459]
Simple Western: beta Tubulin Antibody [NB100-56459] - Image shows a specific band for beta Tubulin in 0.5 mg/mL of Human Brain lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.Applications for beta Tubulin Antibody
Application
Recommended Usage
Immunohistochemistry
1:100 - 1:1000
Immunohistochemistry-Paraffin
5 - 10 ug/mL
Simple Western
1:50
Western Blot
0.1 - 1 ug/mL
Application Notes
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: beta Tubulin
Long Name
Tubulin beta chain
Alternate Names
OK/SW-cl.56, TUBB, TUBB5
Gene Symbol
TUBB2A
UniProt
Additional beta Tubulin Products
Product Documents for beta Tubulin Antibody
Product Specific Notices for beta Tubulin Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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