BID Antibody (002)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-89515
Recombinant Monoclonal Antibody
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 002
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was obtained from a rabbit immunized with purified, recombinant Human BID (Uniprot#: P55957-1; Met1-Asp195).
Specificity
Has cross-reactivity in ELISA with: Mouse BID
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Scientific Data Images for BID Antibody (002)
Western Blot: BID Antibody (002) [NBP2-89515]
Western Blot: BID Antibody (002) [NBP2-89515] - Lane A: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane.Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:22 kDa Observed band size:22 kDaImmunoprecipitation: BID Antibody (002) [NBP2-89515]
Immunoprecipitation: BID Antibody (002) [NBP2-89515] - Lane A:0.5 mg Jurkat Whole Cell Lysate Lane B:0.5 mg RAW264.7 Whole Cell Lysate 2 uL anti-BID rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-BID rabbit polyclonal antibody,at 1:200 dilution Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 22 kDa Observed band size: 22 kDaApplications for BID Antibody (002)
Application
Recommended Usage
ELISA
1:5000-1:10000
Immunoprecipitation
0.5-2 uL/mg of lysate
Western Blot
1:500-1:2000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
0.2 um filtered solution in PBS
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: BID
Long Name
BH3 Interacting Domain Death Agonist
Alternate Names
apoptic death agonist, BH3 interacting domain death agonist, BH3-interacting domain death agonist, BID isoform ES(1b), BID isoform L(2), BID isoform Si6, desmocollin type 4, FP497, Human BID coding sequence, MGC15319, MGC42355, p22 BID, tbid
Gene Symbol
BID
Additional BID Products
Product Documents for BID Antibody (002)
Product Specific Notices for BID Antibody (002)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...