BID Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB500-245
![Western Blot: BID Antibody [NB500-245]](https://resources.bio-techne.com/images/products/BID-Antibody-Western-Blot-NB500-245-img0001.jpg "Western Blot: BID Antibody [NB500-245]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
1.0 mg/ml
Product Specifications
Immunogen
The immunogen this antibody was made to, maps to a region between residues 60 and 120 of human BH3 interacting domain death agonist using the number given in entry NP_932070.1 (GeneID 637). The epitope is found in isoforms 1 and 2 (but not isoform 3) of human BID.
Localization
Isoform 1, isoform 3: Cytoplasm. Isoform 2: Mitochondrion; mitochondrial membrane.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for BID Antibody
Western Blot: BID Antibody [NB500-245]
Western Blot: BID Antibody [NB500-245] - Detection of human BID by Western blot. Samples: HeLa whole cell extracts.![Immunohistochemistry-Paraffin: BID Antibody [NB500-245]](https://resources.bio-techne.com/images/products/BID-Antibody-Immunohistochemistry-NB500-245-img0004.jpg "Immunohistochemistry-Paraffin: BID Antibody [NB500-245]")
Immunohistochemistry-Paraffin: BID Antibody [NB500-245]
Immunohistochemistry-Paraffin: BID Antibody [NB500-245] - Section of human colon carcinoma. Antibody: Affinity purified rabbit anti-BID used at a dilution of 1:1,000 (1ug/ml). Detection: DABApplications for BID Antibody
Application
Recommended Usage
Immunohistochemistry
1:500 - 1:2000
Immunohistochemistry-Paraffin
1:500 - 1:2000
Western Blot
1:1000-1:10000
Application Notes
By Western Blot, this antibody recognizes a band at ~22 kDa, representing BID protein. *The investigator should determine the optimal working dilution for a specific application.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: BID
Long Name
BH3 Interacting Domain Death Agonist
Alternate Names
apoptic death agonist, BH3 interacting domain death agonist, BH3-interacting domain death agonist, BID isoform ES(1b), BID isoform L(2), BID isoform Si6, desmocollin type 4, FP497, Human BID coding sequence, MGC15319, MGC42355, p22 BID, tbid
Entrez Gene IDs
637 (Human)
Gene Symbol
BID
UniProt
Additional BID Products
Product Documents for BID Antibody
Product Specific Notices for BID Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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