Biliverdin Reductase B/BLVRB Antibody (009)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-90188
Recombinant Monoclonal Antibody
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
ELISA, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 009
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for Biliverdin Reductase B/BLVRB Antibody (009)
Immunogen
This antibody was obtained from a rabbit immunized with purified, recombinant Human Biliverdin Reductase B/BLVRB (Uniprot#: P30043; Ala2-Gln206).
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Scientific Data Images for Biliverdin Reductase B/BLVRB Antibody (009)
Western Blot: Biliverdin Reductase B/BLVRB Antibody (009) [NBP2-90188]
Western Blot: Biliverdin Reductase B/BLVRB Antibody (009) [NBP2-90188] - Lane A: Hela Whole Cell LysateLane B: HepG2 Whole Cell LysateLane C: K562 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:22 kDaObserved band size:22 kDaImmunoprecipitation: Biliverdin Reductase B/BLVRB Antibody (009) [NBP2-90188]
Immunoprecipitation: Biliverdin Reductase B/BLVRB Antibody (009) [NBP2-90188] - Lane A:0.5 mg Hela Whole Cell LysateLane B:0.5 mg HepG2 Whole Cell LysateLane C:0.5 mg K562 Whole Cell LysateLane D:0.5 mg Caco-2 Whole Cell Lysate2 uL anti-BLVRB rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-BLVRB rabbit monoclonal antibody,at 1:100 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 22 kDaObserved band size: 22 kDaApplications for Biliverdin Reductase B/BLVRB Antibody (009)
Application
Recommended Usage
ELISA
1:5000-1:10000
Immunoprecipitation
1-4 uL/mg of lysate
Western Blot
1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
0.2 um filtered solution in PBS
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Biliverdin Reductase B/BLVRB
Alternate Names
BLVRB, BVRB, FLR, GHBP
Gene Symbol
BLVRB
Additional Biliverdin Reductase B/BLVRB Products
Product Documents for Biliverdin Reductase B/BLVRB Antibody (009)
Product Specific Notices for Biliverdin Reductase B/BLVRB Antibody (009)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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