Bim Antibody (3C5) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-80102
![Western Blot: Bim Antibody (3C5)BSA Free [NBP2-80102]](https://resources.bio-techne.com/images/products/Bim-Antibody-3C5-Western-Blot-NBP2-80102-img0002.jpg "Western Blot: Bim Antibody (3C5)BSA Free [NBP2-80102]")
Conjugate
Catalog #
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Validated:
Human, Mouse, Rat, Canine, Monkey
Applications
ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Knockout Validated, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2a Kappa Clone # 3C5
Format
BSA Free
Concentration
1 mg/ml
Product Summary for Bim Antibody (3C5) - BSA Free
Immunogen
Synthetic peptide corresponding to aa 20-40 of mouse BimL.
Specificity
Recognizes an epitope within aa 20-40 of human, mouse, rat, monkey and dog BimL (~19kDa), BimEL (~23kDa), and BimS (~16kDa). Also recognizes the six novel isoforms of human Bim, generated by alternative splicing and lacking the C-terminal hydrophobic region.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2a Kappa
Scientific Data Images for Bim Antibody (3C5) - BSA Free
Western Blot: Bim Antibody (3C5)BSA Free [NBP2-80102]
Western Blot: Bim Antibody (3C5) [NBP2-80102] - Detecting EE mouse BimS, BimL, BimEL and EE human, BimSS, BimS, BimLS, BimL, BimELS, BimEL. Probing with an anti-Hsp-70 mAb was used as a loading control for all blots. B) Western blot using anti-Bim, mAb (3C5) detecting endogenous Bim expression in mouse tissues but not in Bim knockout mice. Bim protein was revealed in lysates from mouse tissues (5 x 106/lane) and detection by ECL. Probing with an anti-Hsp-70 mAb was used as a loading control.![Flow Cytometry: Bim Antibody (3C5) - BSA Free [NBP2-80102]](https://resources.bio-techne.com/images/products/Bim-Antibody-3C5-Flow-Cytometry-NBP2-80102-img0001.jpg "Flow Cytometry: Bim Antibody (3C5) - BSA Free [NBP2-80102]")
Flow Cytometry: Bim Antibody (3C5) - BSA Free [NBP2-80102]
Flow Cytometry: Bim Antibody (3C5) [NBP2-80102] - Data in permeabilized and fixed transfected cells using anti-Bim, mAb (3C5) anti-EE (positive control) or secondary antibody alone (negative control). Staining was visualised by FITC-conjugated goat anti-rat or anti-mouse IgG antibodies.Applications for Bim Antibody (3C5) - BSA Free
Application
Recommended Usage
Immunoprecipitation
1-2 ug/ml
Western Blot
1-5 ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS containing 25% glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Bim
Alternate Names
BAM, bcl-2 interacting mediator of cell death, bcl-2 interacting protein Bim, Bcl2-interacting mediator of cell death, Bcl2-L-11, BCL2-like 11 (apoptosis facilitator), bcl-2-like protein 11, bcl-2-related ovarian death agonist, BIM-alpha6, BIM-beta6, BIM-beta7, BIMBOD, BimEL, BimL
Gene Symbol
BCL2L11
Additional Bim Products
Product Documents for Bim Antibody (3C5) - BSA Free
Product Specific Notices for Bim Antibody (3C5) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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