BIN3 Antibody (3A4)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-21676
Key Product Details
Species Reactivity
Human, Mouse
Applications
ELISA, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 3A4
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
BIN3 Antibody (3A4) was produced in mouse by repeated immunizations with a full length human BIN3 recombinant protein followed by hybridoma development. (Uniprot: Q9NQY0)
Reactivity Notes
Cross-reactivity with BIN3 from other sources has not been determined
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Description
This antibody was purified from clarified mouse ascetic fluid by Protein A chromatography followed by extensive dialysis against the buffer stated above
Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Scientific Data Images for BIN3 Antibody (3A4)
![Western Blot: BIN3 Antibody (3A4) [NBP2-21676]](https://resources.bio-techne.com/images/products/BIN3-Antibody-3A4-Western-Blot-NBP2-21676-img0001.jpg "Western Blot: BIN3 Antibody (3A4) [NBP2-21676]")
Western Blot: BIN3 Antibody (3A4) [NBP2-21676]
Western Blot: BIN3 Antibody (3A4) [NBP2-21676] - Mouse Anti-BIN3 antibody. Lane 1: MEF cells wild type. Lane 2: MEF cells null mice. Load: 35 ug per lane. BIN3 antibody overnight at 4C. Secondary antibody: Dye800 mouse secondary antibody for 45 min at RT. Block: 5% Blocking Buffer overnight at 4C. Predicted/Observed size: 29.7 kDa, 30 kDa for BIN-3. Other band: non-specific.
BIN3 Antibody (3A4)
Western Blot of Mouse anti-BIN3 antibody. Lane 1: MEF lysate BIN3 +/-. Lane 2: MEF lysate BIN3 -/-. Load: 35 ug per lane. Primary antibody: BIN-3 antibody at 1:400 for overnight at 4C. Secondary antibody: IRDye800(TM) mouse secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 29.7 kDa, 31 kDa for BIN 3. Other band(s): * non-specifics.Applications for BIN3 Antibody (3A4)
Application
Recommended Usage
ELISA
1:5000-1:50000
Immunoprecipitation
10-100 ul
Western Blot
1:500-1:1500
Application Notes
This product has been tested for use in ELISA, Western Blot, and IP. Specific conditions for reactivity should be optimized by the end user.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
Background: BIN3
Additional BIN3 Products
Product Documents for BIN3 Antibody (3A4)
Product Specific Notices for BIN3 Antibody (3A4)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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