Biotin Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB10915
Recombinant Monoclonal Antibody.
Key Product Details
Species Reactivity
Multi-Species
Applications
Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Simple Western, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 2758A
Product Specifications
Immunogen
Biotin compound
Specificity
Detects Biotin in direct ELISAs.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Biotin Antibody
Detection of Human Biotin by Western Blot.
Western blot shows recombinant Human VEGF 165 aa1-191/Avitag, biotinylated via BirA (AVI293). PVDF membrane was probed with 2 µg/mL of Rabbit Anti-Biotin Monoclonal Antibody (Catalog # MAB10915) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (HAF008). Specific bands were detected for Biotin at approximately various kDa (as indicated). This experiment was conducted under reducing conditions.
Detection of Biotin in Human Testis.
Biotin was detected in immersion fixed paraffin-embedded sections of human testis using Rabbit Anti-Biotin Monoclonal Antibody (Catalog # MAB10915) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in Vimentin stained cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Detection of Mouse Biotin by Simple WesternTM.
Simple Western lane view shows lysates of biotinylated mouse IgG, loaded at 0.2 mg/mL. Specific bands were detected for Biotin at various kDa (as indicated) using 20 µg/mL of Rabbit Anti-Biotin Monoclonal Antibody (Catalog # MAB10915) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Applications for Biotin Antibody
Application
Recommended Usage
Flow Cytometry
0.25 µg/106 cells
Sample: Human PBMC stained with biotinylated antibody
Sample: Human PBMC stained with biotinylated antibody
Immunocytochemistry
1-8 µg/mL
Sample: Immersion fixed HeLa cells
Sample: Immersion fixed HeLa cells
Immunohistochemistry
1-3 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human testis
Sample: Immersion fixed paraffin-embedded sections of human testis
Simple Western
20 µg/mL
Sample: Biotinylated mouse IgG
Sample: Biotinylated mouse IgG
Western Blot
2 µg/mL
Sample: Recombinant Human VEGF 165 aa1-191/Avitag, biotinylated via BirA
Sample: Recombinant Human VEGF 165 aa1-191/Avitag, biotinylated via BirA
Formulation, Preparation, and Storage
Purification
Protein A or G purified from cell culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Biotin
Long Name
Biotin
Alternate Names
Vitamin B4
Additional Biotin Products
Product Documents for Biotin Antibody
Product Specific Notices for Biotin Antibody
For research use only
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