Biotin Antibody (BTN/8672R) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-20861
Recombinant Monoclonal Antibody
Conjugate
Catalog #
Key Product Details
Species Reactivity
All Species
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, In-situ Hybridization, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Kappa Clone # BTN/8672R
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Biotinylated sheep immunoglobulin
Localization
Depends on the target
Specificity
This monoclonal antibody is highly specific to biotin and shows no cross-reaction with other structurally related compounds It has a very high affinity for biotin and is excellent for use in various amplification techniques In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining Anti-biotin antibody may be substituted to decrease this noise
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG Kappa
Description
Positive Controls: Biotinylated proteins in solution or on tissues
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Non-hazardous. No MSDS required.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Non-hazardous. No MSDS required.
Scientific Data Images for Biotin Antibody (BTN/8672R) - Azide and BSA Free
Biotin Antibody (BTN/8672R)
Formalin-fixed, paraffin-embedded human kidney stained with Biotin antibody (BTN/8672R).HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.Applications for Biotin Antibody (BTN/8672R) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
1-2 ug/million cells
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Immunohistochemistry-Paraffin
1-2 ug/ml
Western Blot
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes. at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
10mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -70C. Avoid freeze-thaw cycles.
Background: Biotin
Long Name
Biotin
Alternate Names
Vitamin B4
Additional Biotin Products
Product Documents for Biotin Antibody (BTN/8672R) - Azide and BSA Free
Product Specific Notices for Biotin Antibody (BTN/8672R) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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