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Biotin Antibody (BTN/8672R) [Allophycocyanin]

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-20861APC

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-20861APC

Key Product Details

Species Reactivity

All Species

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, In-situ Hybridization, Western Blot

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Recombinant Monoclonal Rabbit IgG Kappa Clone # BTN/8672R

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Biotinylated sheep immunoglobulin

Localization

Depends on the target

Specificity

This monoclonal antibody is highly specific to biotin and shows no cross-reaction with other structurally related compounds It has a very high affinity for biotin and is excellent for use in various amplification techniques In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining Anti-biotin antibody may be substituted to decrease this noise

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Kappa

Applications for Biotin Antibody (BTN/8672R) [Allophycocyanin]

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

In-situ Hybridization

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Biotin

Biotin is a water soluble vitamin, generally classified as a B complex vitamin, also called vitamin B4. After the initial discovery of biotin, nearly forty years of research were required to establish it as a vitamin. Biotin is required by all organisms but can only be synthesized by bacteria, yeasts, molds, algae, and some plant species. Biotin is required as prosthetic group of enzymes involved in incorporation of carbon dioxide into organic compounds. Biotin has a MW of 244 Da.

Long Name

Biotin

Alternate Names

Vitamin B4

Additional Biotin Products

Product Documents for Biotin Antibody (BTN/8672R) [Allophycocyanin]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Biotin Antibody (BTN/8672R) [Allophycocyanin]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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