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Biotinidase/BTD Antibody (3B10-2B3)

Novus Biologicals, part of Bio-Techne | Catalog # H00000686-M01

Novus Biologicals, part of Bio-Techne
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H00000686-M01

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 3B10-2B3

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

BTD (AAH12099, 1 a.a. ~ 543 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAHAHIQGGRRAKSRFVVCIMSGARSKLALFLCGCYVVALGAHTGEESVADHHEAEYYVAAVYEHPSILSLNPLALISRQEALELMNQNLDIYEQQVMTAAQKDVQIIVFPEDGIHGFNFTRTSIYPFLDFMPSPQVVRWNPCLEPHRFNDTEVLQRLSCMAIRGDMFLVANLGTKEPCHSSDPRCPKDGRYQFNTNVVFSNNGTLVDRYRKHNLYFEAAFDVPLKVDLITFDTPFAGRFGIFTCFDILFFDPAIRVLRDYKVKHVVYPTAWMNQLPLLAAIEIQKAFAVAFGINVLAANVHHPVLGMTGSGIHTPLESFWYHDMENPKSHLIIAQVAKNPVGLIGAENATGETDPSHSKFLKILSGDPYCEKDAQEVHCDEATKWNVNAPPTFHSEMMYDNFTLVPVWGKEGYLHVCSNGLCCYLLYERPTLSKELYALGVFDGLHTVHGTYYIQVCALVRCGGLGFDTCGQEITEATGIFEFHLWGNFSTSYIFPLFLTSGMTLEVPDQLGWENDHYFLRKSRLSSGLVTAALYGRLYERD

Specificity

BTD - biotinidase

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Biotinidase/BTD Antibody (3B10-2B3)

Western Blot: Biotinidase/BTD Antibody (3B10-2B3) [H00000686-M01]

Western Blot: Biotinidase/BTD Antibody (3B10-2B3) [H00000686-M01]

Western Blot: Biotinidase/BTD Antibody (3B10-2B3) [H00000686-M01] - Analysis of BTD expression in transfected 293T cell line by BTD monoclonal antibody (M01), clone 3B10-2B3.Lane 1: BTD transfected lysate(61.1 KDa).Lane 2: Non-transfected lysate.
Immunoprecipitation: Biotinidase/BTD Antibody (3B10-2B3) [H00000686-M01]

Immunoprecipitation: Biotinidase/BTD Antibody (3B10-2B3) [H00000686-M01]

Immunoprecipitation: Biotinidase/BTD Antibody (3B10-2B3) [H00000686-M01] - Analysis of BTD transfected lysate using anti-BTD monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with BTD MaxPab rabbit polyclonal antibody.

Applications for Biotinidase/BTD Antibody (3B10-2B3)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Biotinidase/BTD

Biotinidase functions to recycle biotin in the body by cleaving biocytin (biotin-epsilon-lysine), a normal product of carboxylase degradation, resulting in regeneration of free biotin. Biotinidase has also been shown to have biotinyl-transferase activity. Defects in the biotinidase gene cause multiple carboxylase deficiency.

Alternate Names

Biotinase, BTD

Entrez Gene IDs

686 (Human)

Gene Symbol

BTD

OMIM

253260 (Human)

Additional Biotinidase/BTD Products

Product Documents for Biotinidase/BTD Antibody (3B10-2B3)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Biotinidase/BTD Antibody (3B10-2B3)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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