BLIMP1/PRDM1 Antibody (3H2-E8) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB600-235

Novus Biologicals, part of Bio-Techne
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NB600-235
NB600-235SS
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Porcine

Cited:

Human, Mouse

Applications

Validated:

Chromatin Immunoprecipitation (ChIP), ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

Block/Neutralize, Flow Cytometry, IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 3H2-E8

Format

BSA Free

Concentration

1 mg/ml

View all BLIMP1/PRDM1 Antibodies »

Product Specifications

Immunogen

A fragment of mouse Blimp-1 corresponding to residues 199-409. [UniProt# Q60636]

Reactivity Notes

Porcine usage reported in customer reviews

Localization

Nuclear

Marker

Plasma Cell Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for BLIMP1/PRDM1 Antibody (3H2-E8) - BSA Free

Western Blot: BLIMP1/PRDM1 Antibody (3H2-E8)BSA Free [NB600-235]

Western Blot: BLIMP1/PRDM1 Antibody (3H2-E8)BSA Free [NB600-235]

Western Blot: BLIMP1/PRDM1 Antibody (3H2-E8) - BSA Free [NB600-235] - BLIMP1/PRDM1 Antibody (3H2-E8) [NB600-235] - The effect of IL-21 and CD40L exposure on MEC1 and MEC2 cells. Expression of EBNA-2 and LMP-1 in IL-21 treated cells. Expression of EBNA-2, LMP-1 and Blimp-1 by immunoblotting; positive control: CBM1-Ral-STO, negative control: Ramos. 1.5x105 cells were loaded in the control lanes and 5x105 were loaded in both untreated and IL-21 treated MEC1 and MEC2 lanes. Note low expression of EBNA-2 and high expression of LMP-1 after IL-21 treatment and induction of Blimp-1 after IL-21 treatment. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0106008), licensed under a CC-BY license.
Immunocytochemistry/ Immunofluorescence: BLIMP1/PRDM1 Antibody (3H2-E8) - BSA Free [NB600-235]

Immunocytochemistry/ Immunofluorescence: BLIMP1/PRDM1 Antibody (3H2-E8) - BSA Free [NB600-235]

Immunocytochemistry/Immunofluorescence: BLIMP1/PRDM1 Antibody (3H2-E8) [NB600-235] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-BLIMP1 (3H2-E8) at 10 ug/ml overnight at 4C and detected with an anti-mouse IgG Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Immunohistochemistry-Paraffin: BLIMP1/PRDM1 Antibody (3H2-E8) - BSA Free [NB600-235]

Immunohistochemistry-Paraffin: BLIMP1/PRDM1 Antibody (3H2-E8) - BSA Free [NB600-235]

Immunohistochemistry-Paraffin: BLIMP1/PRDM1 Antibody (3H2-E8) [NB600-235] - Analysis using paraffin-embedded human tonsil tissue with BLIMP1/PRDM1 antibody at dilution of 1:50. Detection is completed through VC001 (DAB) and Counterstained by hematoxylin; labeling is predominantly in germinal centers.
Images may not be copied, printed or otherwise disseminated without express written permission of Novus Biologicals a bio-techne brand.
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Applications for BLIMP1/PRDM1 Antibody (3H2-E8) - BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation (ChIP)

1ug antibody/ 10 ug protein

ELISA

1:100-1:2000

Flow Cytometry

8 ug/ml where cells are used at 2*10^6/mL

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunohistochemistry

1:200

Immunohistochemistry-Frozen

reported in scientific literature

Immunohistochemistry-Paraffin

1:200

Western Blot

1:1000
Application Notes
By WB, this antibody recognizes a band at ~98 kDa and may recognize one at ~80 kDa (the beta form). Antigen retrieval is recommended (EDTA buffer, microwave) prior to IHC on paraffin tissues. This antibody demonstrates nuclear staining. For IHC and ICC/IF a dilution of 1:200 is recommended with tyramide amplification.

Reviewed Applications

Read 2 reviews rated 3.5 using NB600-235 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: BLIMP1/PRDM1

BLIMP-1 (B lymphocyte-induced maturation protein) is a nuclear zinc-finger containing transcriptional repressor which primarily controls the terminal differentiation of mature B cells to plasma cells. BLIMP-1 is also involved in macrophage and T-cell population homeostasis/differentiation. It is also called positive regulatory domain I-binding factor-1 (PRDI-BF1) or PR (PRDI-BF1-RIZ) domain zinc finger protein 1 (PRDM1) and its first human homolog, PRDI-BF1, was identified by its ability to bind to the PRDI element on the IFN-beta promoter leading to inhibition of virus-mediated IFN-beta production. BLIMP-1 contains N-terminal PR/SET domain and five C2H2 zinc fingers located near its C-terminus that mediate DNA binding, nuclear import and recruitment of histone modifying enzymes, and these activities enable BLIMP-1 to control cell-fate decisions in the embryo and govern tissue homeostasis in multiple cell types in the adult organism. BLIMP-1 recruits chromatin-modifying enzymes including HDACs and methyltransferases, and some of its target genes include c-Myc, CIITA, Pax5, Spi-B, and Id3. BLIMP-1 sumoylation at Lys-816 by PIAS1 augments transcriptional repressor activity, and is critical for plasma cell differentiation. Deletion mutation of BLIMP-1 is frequently observed in several tumor types including lymphoid malignancies, and has been proposed to be a candidate for tumor supression research.

Long Name

B Lymphocyte Induced Maturation Protein

Alternate Names

PRDM1, ZNFPR1A1

Gene Symbol

PRDM1

Additional BLIMP1/PRDM1 Products

Product Documents for BLIMP1/PRDM1 Antibody (3H2-E8) - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for BLIMP1/PRDM1 Antibody (3H2-E8) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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