BLIMP1/PRDM1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-51991
Key Product Details
Species Reactivity
Validated:
Human, Rat
Cited:
Rat
Predicted:
Bovine (100%), Canine (100%), Mouse (100%), Porcine (100%). Backed by our 100% Guarantee.
Applications
Validated:
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot
Cited:
Flow Cytometry
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
0.5 mg/ml
Product Specifications
Immunogen
Peptide with sequence C-DISDNADRLEDVED, from the internal region of the protein sequence according to NP_001189.2; NP_878911.1.
Epitope
C-DISDNADRLEDVED
Reactivity Notes
Rat reactivity reported in scientific literature (PMID:32981181)
Specificity
This antibody is expected to recognize both reported isoforms (NP_001189.2; NP_878911.1).
Marker
Plasma Cell Marker
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for BLIMP1/PRDM1 Antibody
![Western Blot: BLIMP1/PRDM1 Antibody [NBP1-51991]](https://resources.bio-techne.com/images/products/BLIMP1-PRDM1-Antibody-Western-Blot-NBP1-51991-img0001.jpg "Western Blot: BLIMP1/PRDM1 Antibody [NBP1-51991]")
Western Blot: BLIMP1/PRDM1 Antibody [NBP1-51991]
Western Blot: BLIMP1/PRDM1 Antibody [NBP1-51991] - Staining of A431 (A), (2 ug/mL) K562 (B) and (1 ug/mL) Nuclear K562 (C) cell lysate (35 ug protein in RIPA buffer). Antibody at 0.1 ug/mL. Detected by chemiluminescence.![Flow Cytometry: BLIMP1/PRDM1 Antibody [NBP1-51991]](https://resources.bio-techne.com/images/products/BLIMP1-PRDM1-Antibody-Flow-Cytometry-NBP1-51991-img0003.jpg "Flow Cytometry: BLIMP1/PRDM1 Antibody [NBP1-51991]")
Flow Cytometry: BLIMP1/PRDM1 Antibody [NBP1-51991]
Flow Cytometry: BLIMP1/PRDM1 Antibody [NBP1-51991] - Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.![Immunofluorescence: BLIMP1/PRDM1 Antibody [NBP1-51991]](https://resources.bio-techne.com/images/products/BLIMP1-PRDM1-Antibody-Immunofluorescence-NBP1-51991-img0002.jpg "Immunofluorescence: BLIMP1/PRDM1 Antibody [NBP1-51991]")
Immunofluorescence: BLIMP1/PRDM1 Antibody [NBP1-51991]
Immunofluorescence: BLIMP1/PRDM1 Antibody [NBP1-51991] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).Applications for BLIMP1/PRDM1 Antibody
Application
Recommended Usage
Flow Cytometry
10 ug/mL
Immunohistochemistry-Paraffin
6 ug/ml
Peptide ELISA
Detection limit 1:1000
Western Blot
0.1 - 2 ug/mL
Application Notes
WB: Approx 90kDa band observed in lysates of cell lines A431 and Nuclear K562, and approx. 100kDa in lysates of cell line K562. An additional faint band of approx 70kDa was also seen in some A431 and K562 lysates (calculated MW of 91.8kDa according to NP_001189.2 isoform 1 and 76.8kD according to NP_878911.1 isoform 2). Primary incubation 1 hour at room temperature.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: BLIMP1/PRDM1
Blimp1 is a nuclear protein with five zinc fingers in its carboxy terminus. The Blimp-1 protein is expressed in, and may be required for terminally differentiated epithelial cells, retinal neurons and other lineage cells. BLIMP-1 has also been implicated in the repression of beta-interferon (IFN-beta) and c-myc gene expression.
Long Name
B Lymphocyte Induced Maturation Protein
Alternate Names
PRDM1, ZNFPR1A1
Gene Symbol
PRDM1
UniProt
Additional BLIMP1/PRDM1 Products
Product Documents for BLIMP1/PRDM1 Antibody
Product Specific Notices for BLIMP1/PRDM1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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