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BMI-1 Antibody (1F2)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26477

Recombinant monoclonal antibody expressed in HEK293F cells
Novus Biologicals, part of Bio-Techne
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NBP3-26477-100ul
NBP3-26477-50ul

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 1F2

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

A synthesized peptide derived from Human BMI-1 [UniProt P35226]

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for BMI-1 Antibody (1F2)

BMI-1 Antibody (1F2)

Immunohistochemistry: BMI-1 Antibody (1F2) [NBP3-26477] -

Immunohistochemistry: BMI-1 Antibody (1F2) [NBP3-26477] - Image of BMI-1 Antibody (1F2) diluted at 1:100 and staining in paraffin-embedded human liver cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
BMI-1 Antibody (1F2)

Immunohistochemistry: BMI-1 Antibody (1F2) [NBP3-26477] -

Immunohistochemistry: BMI-1 Antibody (1F2) [NBP3-26477] - Image of BMI-1 Antibody (1F2) diluted at 1:100 and staining in paraffin-embedded human liver cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
BMI-1 Antibody (1F2)

Western Blot: BMI-1 Antibody (1F2) [NBP3-26477] -

Western Blot: BMI-1 Antibody (1F2) [NBP3-26477] - Positive Western Blot detected in: Hela whole cell lysate, 293 whole cell lysate, K562 whole cell lysate, THP-1 whole cell lysate, U87 whole cell lysate, U251 whole cell lysate.
All lanes: BMI-1 Antibody at 1: 2000
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 37 kDa
Observed band size: 45 kDa

Applications for BMI-1 Antibody (1F2)

Application
Recommended Usage

Immunohistochemistry

1:50-1:200

Western Blot

1:500-1:5000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl and 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: BMI-1

Bmi1 polycomb ring finger oncogene regulates the cell cycle inhibitor genes p16 and p19, with implications in hematopoiesis, skeletal patterning, neurological functions, and development of the cerebellum. Bmi 1 has been shown to induce telomerase activity and immortalize human mammary epithelial cells, and extend the replicative life span of human fibroblasts by suppressing the p16-dependent senescence pathway. Bmi-1 is also required for efficient self-renewing cell division in adult hematopoietic and neural stem cells.

Aberrant expression of Bmi-1 has been found in several types of cancer in the bladder, skin, blood, prostate, breast, ovaries, andcolon. Its overexpression is especially pronounced in mantle cell lymphomas. Thereforem Bmi1 antibodies will be useful tools for many cancer development studies.

Long Name

B Lymphoma Mo-MLV Insertion Region 1

Alternate Names

BMI1, PCGF4, RNF51

Gene Symbol

BMI1

Additional BMI-1 Products

Product Documents for BMI-1 Antibody (1F2)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for BMI-1 Antibody (1F2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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