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BMP-4 Antibody (19B7)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26476

Recombinant monoclonal antibody expressed in HEK293F cells
Novus Biologicals, part of Bio-Techne
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NBP3-26476-100ul
NBP3-26476-50ul

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 19B7

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

A synthesized peptide derived from Human BMP-4 [UniProt P12644]

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for BMP-4 Antibody (19B7)

BMP-4 Antibody (19B7)

Western Blot: BMP-4 Antibody (19B7) [NBP3-26476] -

Western Blot: BMP-4 Antibody (19B7) [NBP3-26476] - Positive Western Blot detected in: Hela whole cell lysate.
All lanes: BMP-4 Antibody at 1: 2000
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 47 kDa
Observed band size: 36-55 kDa
BMP-4 Antibody (19B7)

Immunocytochemistry/Immunofluorescence: BMP-4 Antibody (19B7) [NBP3-26476] -

Immunocytochemistry/Immunofluorescence: BMP-4 Antibody (19B7) [NBP3-26476] - Staining of Hela cell with BMP-4 Antibody (19B7) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 508-conjugated Goat Anti-Rabbit IgG (H+L).

Applications for BMP-4 Antibody (19B7)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl and 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: BMP-4

Bone morphogenetic proteins were originally identified by an ability of demineralized bone extract to induce endochondral osteogenesis in vivo in an extraskeletal site. Through molecular cloning, 7 BMP cDNAs, designated BMP1 through BMP7, have been recovered. BMP2 through BMP7, are members of the transforming growth factor-beta superfamily of regulatory molecules. Transcript variants utilizing alternative promoters and alternative non-coding first exons have been described in the literature.

Long Name

Bone Morphogenetic Protein 4

Alternate Names

BMP2B, BMP2B1, BMP4, MCOPS6, OFC11

Gene Symbol

BMP4

Additional BMP-4 Products

Product Documents for BMP-4 Antibody (19B7)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for BMP-4 Antibody (19B7)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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