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BMP-4 Antibody (3C11H8) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-52423

Novus Biologicals, part of Bio-Techne
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NBP2-52423
NBP2-52423-0.025mg

Key Product Details

Species Reactivity

Validated:

Human, Rat

Applications

CyTOF-ready, ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 3C11H8

Format

BSA Free

Concentration

1 mg/ml

Product Summary for BMP-4 Antibody (3C11H8) - BSA Free

Immunogen

Purified recombinant fragment of human BMP-4 (AA: 277-408) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

46.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for BMP-4 Antibody (3C11H8) - BSA Free

Western Blot: BMP-4 Antibody (3C11H8)BSA Free [NBP2-52423]

Western Blot: BMP-4 Antibody (3C11H8)BSA Free [NBP2-52423]

Western Blot: BMP-4 Antibody (3C11H8) [NBP2-52423] - Analysis using BMP4 mAb against human BMP4 (AA: 277-408) recombinant protein. (Expected MW is 41 kDa).
Immunocytochemistry/ Immunofluorescence: BMP-4 Antibody (3C11H8) - BSA Free [NBP2-52423]

Immunocytochemistry/ Immunofluorescence: BMP-4 Antibody (3C11H8) - BSA Free [NBP2-52423]

Immunocytochemistry/Immunofluorescence: BMP-4 Antibody (3C11H8) [NBP2-52423] - Analysis of Hela cells using BMP-4 mouse mAb. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Western Blot: BMP-4 Antibody (3C11H8)BSA Free [NBP2-52423]

Western Blot: BMP-4 Antibody (3C11H8)BSA Free [NBP2-52423]

Western Blot: BMP-4 Antibody (3C11H8) [NBP2-52423] - Analysis using BMP4 mAb against HEK293 (1) and BMP4 (AA: 277-408)-hIgGFc transfected HEK293 (2) cell lysate.

Applications for BMP-4 Antibody (3C11H8) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Immunocytochemistry/ Immunofluorescence

1:100-1:500

Western Blot

1:500-1:2000
Application Notes
This antibody is Cytof ready.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: BMP-4

Bone morphogenetic proteins were originally identified by an ability of demineralized bone extract to induce endochondral osteogenesis in vivo in an extraskeletal site. Through molecular cloning, 7 BMP cDNAs, designated BMP1 through BMP7, have been recovered. BMP2 through BMP7, are members of the transforming growth factor-beta superfamily of regulatory molecules. Transcript variants utilizing alternative promoters and alternative non-coding first exons have been described in the literature.

Long Name

Bone Morphogenetic Protein 4

Alternate Names

BMP2B, BMP2B1, BMP4, MCOPS6, OFC11

Gene Symbol

BMP4

Additional BMP-4 Products

Product Documents for BMP-4 Antibody (3C11H8) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for BMP-4 Antibody (3C11H8) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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