Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI6B7

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all BMP-4 Antibodies »

Product Summary for BMP-4 Antibody (OTI6B7) - Azide and BSA Free

Immunogen

Recombinant fragment expressed in E.coli corresponding to amino acids 293-408 of human BMP4

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

46.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for BMP-4 Antibody (OTI6B7) - Azide and BSA Free

Western Blot: BMP-4 Antibody (OTI6B7)Azide and BSA Free [NBP2-70256]

Western Blot: BMP-4 Antibody (OTI6B7)Azide and BSA Free [NBP2-70256]

Western Blot: BMP-4 Antibody (OTI6B7) - Azide and BSA Free [NBP2-70256] - BMP4 antibody ( 6B7 ) at 1:100000 dilution + Recombinant human BMP4.
Immunocytochemistry/ Immunofluorescence: BMP-4 Antibody (OTI6B7) - Azide and BSA Free [NBP2-70256]

Immunocytochemistry/ Immunofluorescence: BMP-4 Antibody (OTI6B7) - Azide and BSA Free [NBP2-70256]

Immunocytochemistry/Immunofluorescence: BMP-4 Antibody (OTI6B7) - Azide and BSA Free [NBP2-70256] - Staining of HeLa cells transiently transfected by pCMV6-ENTRY BMP4.

Applications for BMP-4 Antibody (OTI6B7) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:100000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: BMP-4

Bone morphogenetic proteins were originally identified by an ability of demineralized bone extract to induce endochondral osteogenesis in vivo in an extraskeletal site. Through molecular cloning, 7 BMP cDNAs, designated BMP1 through BMP7, have been recovered. BMP2 through BMP7, are members of the transforming growth factor-beta superfamily of regulatory molecules. Transcript variants utilizing alternative promoters and alternative non-coding first exons have been described in the literature.

Long Name

Bone Morphogenetic Protein 4

Alternate Names

BMP2B, BMP2B1, BMP4, MCOPS6, OFC11

Gene Symbol

BMP4

Additional BMP-4 Products

Product Documents for BMP-4 Antibody (OTI6B7) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for BMP-4 Antibody (OTI6B7) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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