BNIP3 Antibody (ANa40) [Janelia Fluor® 525]

Novus Biologicals, part of Bio-Techne | Catalog # NB100-1543JF525

Submit a review

Product Datasheet / COA / SDS

View Available Conjugates View Available Formulations

Conjugate

Catalog #

Alexa Fluor 350

NB100-1543AF350

Alexa Fluor 405

NB100-1543AF405

Alexa Fluor 488

NB100-1543AF488

Alexa Fluor 532

NB100-1543AF532

Alexa Fluor 594

NB100-1543AF594

Alexa Fluor 647

NB100-1543AF647

Alexa Fluor 700

NB100-1543AF700

Alexa Fluor 750

NB100-1543AF750

Biotin

NB100-1543B

CoraFluor 1

NB100-1543CL1

DyLight 350

NB100-1543UV

DyLight 405

NB100-1543V

DyLight 488

NB100-1543G

DyLight 550

NB100-1543R

DyLight 594

NB100-1543DL594

DyLight 650

NB100-1543C

DyLight 680

NB100-1543FR

DyLight 755

NB100-1543IR

FITC

NB100-1543F

HRP

NB100-1543H

Janelia Fluor 549

NB100-1543JF549

Janelia Fluor 585

NB100-1543JF585

Janelia Fluor 635

NB100-1543JF635

Janelia Fluor 646

NB100-1543JF646

Janelia Fluor 669

NB100-1543JF669

Forumulation

Catalog #

BSA Free

NB100-1543

Product Summary
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Label

Janelia Fluor 525

Antibody Source

Monoclonal Mouse IgG_2B Clone # ANa40

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all BNIP3 Antibodies »

Product Summary for BNIP3 Antibody (ANa40) [Janelia Fluor® 525]

Immunogen

Recombinant fragment (Residues 1-163 of Human BNIP3).

Epitope

The epitope recognized by the antibody resides within amino acids 112-124 of human BNIP3 molecule.

Reactivity Notes

Not yet tested in other species.

Localization

Mitochondrial and Nuclear membrane

Specificity

BNIP3 (ANa40)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2B

Applications for BNIP3 Antibody (ANa40) [Janelia Fluor® 525]

Application

Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Application Notes

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: BNIP3

BCL2/adenovirus E1B 19kD protein-interacting protein 3 (BNIP3) is a mitochondrial protein critical for apoptotic events in response to adenovirus infection. It has been implicated in causing a necrotic-like cell death pattern in cells, induced by CD47. This effect has been seen in many cancer cells, and its mis-regulation causes tumor cells to be impervious to normal autophagic events. The tumor suppressor p53 represses the expression of BNIP3, often as a consequence of hypoxic micro-environments as seen in tumors. When BNIP3 is correctly expressed and activated, it translocates to the mitochondria and instigates apoptosis.

Long Name

BCL2/Adenovirus E1B 19 Kda Protein-Interacting Protein 3-Like

Alternate Names

Nip3

Gene Symbol

BNIP3

Additional BNIP3 Products

Product Documents for BNIP3 Antibody (ANa40) [Janelia Fluor® 525]

Product Datasheets

Download Product Datasheets

COA

SDS

Product Specific Notices for BNIP3 Antibody (ANa40) [Janelia Fluor® 525]

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations
Reviews