Skip to main content

BNIP3L Antibody (HL1203) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-25384

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
Loading...
NBP3-25384

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (100%), Chicken (100%), Rhesus Macaque (100%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL1203

Format

Azide and BSA Free

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Summary for BNIP3L Antibody (HL1203) - Azide and BSA Free

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human BNIP3L. The exact sequence is proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Zebrafish (80%), Xenopus (86%).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for BNIP3L Antibody (HL1203) - Azide and BSA Free

BNIP3L Antibody (HL1203) - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: BNIP3L Antibody (HL1203) - Azide and BSA Free [NBP3-25384] -

Immunocytochemistry/Immunofluorescence: BNIP3L Antibody (HL1203) - Azide and BSA Free [NBP3-25384] - BNIP3L antibody [HL1203] detects BNIP3L protein at mitochondria by immunofluorescent analysis. Sample: Mock and treated HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: BNIP3L stained by BNIP3L antibody [HL1203] (NBP3-25384) diluted at 1:500. Blue: Fluoroshield with DAPI .
BNIP3L Antibody (HL1203) - Azide and BSA Free

Western Blot: BNIP3L Antibody (HL1203) - Azide and BSA Free [NBP3-25384] -

Western Blot: BNIP3L Antibody (HL1203) - Azide and BSA Free [NBP3-25384] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with BNIP3L antibody [HL1203] (NBP3-25384) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
BNIP3L Antibody (HL1203) - Azide and BSA Free

Immunohistochemistry-Paraffin: BNIP3L Antibody (HL1203) - Azide and BSA Free [NBP3-25384] -

Immunohistochemistry-Paraffin: BNIP3L Antibody (HL1203) - Azide and BSA Free [NBP3-25384] - BNIP3L antibody [HL1203] detects BNIP3L protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissues. BNIP3L stained by BNIP3L antibody [HL1203] (NBP3-25384) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Applications for BNIP3L Antibody (HL1203) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: BNIP3L

Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bcl-2 homology 3 (BH3) domain is a potent death domain. BH3 domain containing pro-apoptotic proteins, including Bad, Bax, Bid, Bik, Hrk, Nip3, and Bim, form a growingsubclass of the Bcl-2 family. A novel BH3 domaincontaining protein was recently identified and designated Bnip3L, Bnip3a, and Nix (for Nip3-like protein X) (Matsushima, M. et al.; Yasuda, M. et al.; Chen, G, et al.).Bnip3L/Bnip3a/Nix is a homolog of the E1B 19K/Bcl-2 binding and pro-apoptotic protein Bnip3. Over expression of Bnip3L induces apoptosis (Yasuda, M. et al.; Chen, G, et al.). Bnip3L interacts withand overcomes suppresses by Bcl-2 and Bcl-xL. Bnip3Lis localized in mitochondria. The messenger RNA of Bnip3L is ubiquitously expressed in human tissues (Matsushima, M. et al.; Yasuda, M. et al.). Bnip3L and Bnip3 form a new subfamily of the pro-apoptotic mitochondrial proteins.

Long Name

BCL2/Adenovirus E1B 19 Kda Protein-Interacting Protein 3-Like

Alternate Names

BNIP3a, Nip3L, Nix

Gene Symbol

BNIP3L

Additional BNIP3L Products

Product Documents for BNIP3L Antibody (HL1203) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for BNIP3L Antibody (HL1203) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Loading...
Loading...
Loading...
Loading...