BNIP3L Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-78264

Novus Biologicals, part of Bio-Techne
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NBP1-78264SS
NBP1-78264
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (100%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.07 mg/ml

View all BNIP3L Antibodies »

Product Specifications

Immunogen

A synthetic peptide made to an internal portion of the human BNIP3L protein (between residues 50-100) [UniProt O60238]

Reactivity Notes

Immunogen has 100% identity to bovine.

Localization

Nucleus envelope. Endoplasmic reticulum. Mitochondrion. Membrane; Single-pass membrane protein.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for BNIP3L Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: BNIP3L Antibody - BSA Free [NBP1-78264]

Immunocytochemistry/ Immunofluorescence: BNIP3L Antibody - BSA Free [NBP1-78264]

Immunocytochemistry/Immunofluorescence: BNIP3L Antibody [NBP1-78264] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-BNIP3L (NBP1-78264) at a 1:200 dilution overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin was used as a co-stain at a 1:1000 dilution and detected with and anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Immunohistochemistry: BNIP3L Antibody - BSA Free [NBP1-78264]

Immunohistochemistry: BNIP3L Antibody - BSA Free [NBP1-78264]

Immunohistochemistry: BNIP3L Antibody [NBP1-78264] - Analysis of BNIP3L in mouse prostate using DAB with hematoxylin counterstain.

Applications for BNIP3L Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:300

Immunohistochemistry-Paraffin

1:300
Application Notes
This BNIP3L antibody is useful for Immunocytochemistry/Immunofluorescence and Immunohistochemistry-Paraffin. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS and 30% Glycerol

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.07 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: BNIP3L

BNIP3L/NIX is a member of the BCL2/adenovirus E1B 19 kd-interacting protein (BNIP) family, which is involved in the regulation of apoptosis. Like other BH3-only proteins, NIX is believed to possess tumor suppressing functions; NIX localizes to the mitochondria and interacts with BCL2 and BCL-X in a pro-apoptotic manner. NIX shares a high level of sequence homology with BNIP3, and like BNIP3 activates apoptosis through its C-terminal transmembrane domain but not via its BH3 domain. Hypoxia is believed to be a principle regulator of NIX activity, making NIX a key factor in hypoxia-induced tumor cell death and heart disease. In addition, NIX has been implicated in mitophagy, a specific form of autophagy; due to its dual role, NIX is an interesting target to examine the relationship between apoptosis and autophagy.

Long Name

BCL2/Adenovirus E1B 19 Kda Protein-Interacting Protein 3-Like

Alternate Names

BNIP3a, Nip3L, Nix

Entrez Gene IDs

665 (Human); 12177 (Mouse); 534615 (Bovine)

Gene Symbol

BNIP3L

UniProt

O60238

Additional BNIP3L Products

Product Documents for BNIP3L Antibody - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for BNIP3L Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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