Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunoassay, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 50E1cc

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all BNP Antibodies »

Product Specifications

Immunogen

Hybridoma clone has been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with synthetic human BNP, whole molecule, conjugated with carrier protein.

Epitope

a.a.r. 26-32

Localization

Secreted

Specificity

Detects Human BNP and proBNP.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

14.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for BNP Antibody (50E1cc)

Western Blot: BNP Antibody (50E1cc) [NB200-440]

Western Blot: BNP Antibody (50E1cc) [NB200-440]

Western Blot: proBNP Antibody (50E1) [NB200-440] - Analysis using the Biotin conjugate of NB200-440. Detection of synthetic huBNP in WB after Tricine-SDS-PAGE in reducing conditions.
Western Blot: BNP Antibody (50E1cc) [NB200-440]

Western Blot: BNP Antibody (50E1cc) [NB200-440]

Western Blot: proBNP Antibody (50E1) [NB200-440] - Immunodetection of human synthetic BNP (A), recombinant proBNP (E. coli) (B) and recombinant proBNP (mammalian cell line) (C) in Western blotting by different monoclonal antibodies after Tricine- SDS-PAGE under reducing conditions. A, B. Lanes: 1- MAb 24C511-17, 2- MAb 26E211-22, 3- MAb 2G9, 4- MAb 43B1226-32, 5- MAb 50B726-32, 6- MAb 50E126-32, 7- MAb 57H326-32.
Immunoassay: BNP Antibody (50E1cc) [NB200-440]

Immunoassay: BNP Antibody (50E1cc) [NB200-440]

Immunoassay: proBNP Antibody (50E1) [NB200-440] - Gel-filtration studies of endogenous proBNP and BNP. BNP immunoreactivity measurements in fractions after proteins from plasma of HF patients were separated by gel-filtration on Superdex Peptide column (GEHealthcare). One representative plasma sample. BNP immunoreactivity was measured by 50E126-32-24C511-17 assay (- -). The first peak corresponds to proBNP, the second corresponds to BNP form.

Applications for BNP Antibody (50E1cc)

Application
Recommended Usage

ELISA

1:100-1:2000

Sandwich ELISA

1:100-1:2000

Western Blot

1:100-1:2000
Application Notes
Can be used for proBNP detection in pair with NB200-439.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4)

Preservative

0.09% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: BNP

Brain natriuretic peptide (BNP) circulates in blood as a peptide hormone with natriuretic, vasodilatory and renin inhibitory properties. BNP is secreted predominantly by the left ventricular myocytes in response to volume expansion and pressure overload. BNP belongs to a family of structurally similar peptide hormones, which includes atrial natriuretic peptide (ANP), BNP, C-type natriuretic peptide (CNP) and urodilatin. These peptides are characterized by a common 17 amino acid ring structure with a disulfide bond between two cystein residues. This ring structure shows high homology between different natriuretic peptides (eleven of the 17 amino acid residues are homologous in the ring of each of the natriuretic peptides, see fig. 18). BNP is a 32 amino acid peptide with disulfide bond between the cystein residues Cys10 and Cys26. In earlier studies it has been demonstrated that BNP concentration in blood increases with the severity of congestive heart failure. Quantitative measurement of BNP in blood provides an objective indicator of congestive heart failure severity

Long Name

Brain Natriuretic Peptide

Alternate Names

BNF, NPPB

Entrez Gene IDs

4879 (Human)

Gene Symbol

NPPB

UniProt

P16860

Additional BNP Products

Product Documents for BNP Antibody (50E1cc)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for BNP Antibody (50E1cc)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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