Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin, Protein Array

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # NPPB/4493

Format

Azide and BSA Free

Concentration

1 mg/ml

View all BNP Antibodies »

Product Specifications

Immunogen

Recombinant fragment of human BNP (exact sequence is proprietary) (Uniprot: P16860)

Localization

Secreted

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-13984).

Antibody without azide - store at -20 to -80C. Antibody with azide - store at 2 to 8C. Non-hazardous.

Theoretical molecular weight: 25-36kDa (glycosylated BNP precursor); 12kDa (deglycosylated mature BNP)

Scientific Data Images for BNP Antibody (NPPB/4493) - Azide and BSA Free

Protein Array: BNP Antibody (NPPB/4493) - Azide and BSA Free [NBP3-14020]

Protein Array: BNP Antibody (NPPB/4493) - Azide and BSA Free [NBP3-14020]

Protein Array: BNP Antibody (NPPB/4493) - Azide and BSA Free [NBP3-14020] - Analysis of Protein Array containing more than 19,000 full-length human proteins using BNP Antibody (NPPB/4493).
BNP Antibody (NPPB/4493) - Azide and BSA Free

BNP Antibody (NPPB/4493)

Formalin-fixed, paraffin-embedded human uterusstained with BNP antibody (NPPB/4493). HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.

Applications for BNP Antibody (NPPB/4493) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: BNP

Brain natriuretic peptide (BNP) circulates in blood as a peptide hormone with natriuretic, vasodilatory and renin inhibitory properties. BNP is secreted predominantly by the left ventricular myocytes in response to volume expansion and pressure overload. BNP belongs to a family of structurally similar peptide hormones, which includes atrial natriuretic peptide (ANP), BNP, C-type natriuretic peptide (CNP) and urodilatin. These peptides are characterized by a common 17 amino acid ring structure with a disulfide bond between two cystein residues. This ring structure shows high homology between different natriuretic peptides (eleven of the 17 amino acid residues are homologous in the ring of each of the natriuretic peptides, see fig. 18). BNP is a 32 amino acid peptide with disulfide bond between the cystein residues Cys10 and Cys26. In earlier studies it has been demonstrated that BNP concentration in blood increases with the severity of congestive heart failure. Quantitative measurement of BNP in blood provides an objective indicator of congestive heart failure severity

Long Name

Brain Natriuretic Peptide

Alternate Names

BNF, NPPB

Gene Symbol

NPPB

Additional BNP Products

Product Documents for BNP Antibody (NPPB/4493) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for BNP Antibody (NPPB/4493) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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